Literature DB >> 2468482

A simple, sensitive method of analyzing bacterial ribosomal DNA polymorphism.

N Picard-Pasquier1, M Ouagued, B Picard, P Goullet, R Krishnamoorthy.   

Abstract

A rapid DNA extraction procedure and random primer labelling of Escherichia coli ribosomal RNA with cloned reverse transcriptase have been used to establish a simple and highly sensitive method for studying ribosomal DNA polymorphism in bacteria. Examples of inter- and intraspecies differentiation of bacteria are given and potential applications in bacterial epidemiology and taxonomy are discussed.

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Year:  1989        PMID： 2468482     DOI： 10.1002/elps.1150100306

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  18 in total

1.  Molecular epidemiological analysis of Pseudomonas aeruginosa strains causing failure of antibiotic therapy in cystic fibrosis patients.

Authors:  E Bingen; E Denamur; B Picard; P Goullet; N Lambert-Zechovsky; P Foucaud; J Navarro; J Elion
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-05       Impact factor: 3.267

2.  Genetic diversity of rRNA operons of unrelated Streptococcus agalactiae strains isolated from cerebrospinal fluid of neonates suffering from meningitis.

Authors:  S Chatellier; H Huet; S Kenzi; A Rosenau; P Geslin; R Quentin
Journal:  J Clin Microbiol       Date:  1996-11       Impact factor: 5.948

Review 3.  DNA fingerprinting techniques for microorganisms. A proposal for classification and nomenclature.

Authors:  M Vaneechoutte
Journal:  Mol Biotechnol       Date:  1996-10       Impact factor: 2.695

4.  Evidence for the genetic unrelatedness of nosocomial vancomycin-resistant Enterococcus faecium strains in a pediatric hospital.

Authors:  E H Bingen; E Denamur; N Y Lambert-Zechovsky; J Elion
Journal:  J Clin Microbiol       Date:  1991-09       Impact factor: 5.948

5.  Complexity of Pseudomonas aeruginosa infection in cystic fibrosis: combined results from esterase electrophoresis and rDNA restriction fragment length polymorphism analysis.

Authors:  E Denamur; B Picard; P Goullet; E Bingen; N Lambert; J Elion
Journal:  Epidemiol Infect       Date:  1991-06       Impact factor: 2.451

Review 6.  Use of ribotyping in epidemiological surveillance of nosocomial outbreaks.

Authors:  E H Bingen; E Denamur; J Elion
Journal:  Clin Microbiol Rev       Date:  1994-07       Impact factor: 26.132

7.  Heterogeneity of Shiga toxin-producing Escherichia coli strains isolated from hemolytic-uremic syndrome patients, cattle, and food samples in central France.

Authors:  N Pradel; K Boukhors; Y Bertin; C Forestier; C Martin; V Livrelli
Journal:  Appl Environ Microbiol       Date:  2001-06       Impact factor: 4.792

8.  Genetic diversity of Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis revealed by restriction fragment length polymorphism of the rRNA gene region.

Authors:  C Martin; M A Ichou; P Massicot; A Goudeau; R Quentin
Journal:  J Clin Microbiol       Date:  1995-06       Impact factor: 5.948

9.  Value of plasmid profiling, ribotyping, and detection of IS200 for tracing avian isolates of Salmonella typhimurium and S. enteritidis.

Authors:  Y Millemann; M C Lesage; E Chaslus-Dancla; J P Lafont
Journal:  J Clin Microbiol       Date:  1995-01       Impact factor: 5.948

10.  Genetic characterization of a cryptic genospecies of Haemophilus causing urogenital and neonatal infections.

Authors:  R Quentin; C Martin; J M Musser; N Pasquier-Picard; A Goudeau
Journal:  J Clin Microbiol       Date:  1993-05       Impact factor: 5.948
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