Transphosphorylation of EGFR at Y845 plays an important role in its autophosphorylation and kinase activity.

Epidermal growth factor receptor (EGFR) plays a critical role in various types of human cancer such as lung, breast, brain and colon cancer. However, how EGFR activation is initiated and what type of interaction is linked to its transphosphorylation and autophosphorylation as well as the biological consequences in case of interruption and blockade of these two types of EGFR phosphorylation, remain elusive. In the present study, we provided evidence that EGFR transphosphorylation at Y845 indeed plays an important role in its autophosphorylation and kinase activity. Our results suggest that the modulation of EGFR transphosphorylation may influence its activity and function, indicating a potential target mechanism for treating EGFR-associated diseases and various types of cancer.