| Literature DB >> 24675174 |
Glen DeLoid1, Joel M Cohen1, Tom Darrah2, Raymond Derk3, Liying Rojanasakul3, Georgios Pyrgiotakis4, Wendel Wohlleben5, Philip Demokritou1.
Abstract
The need for accurate in vitro dosimetry remains a major obstacle to the development of cost-effective toxicological screening methods for engineered nanomaterials. An important key to accurate in vitro dosimetry is the characterization of sedimentation and diffusion rates of nanoparticles suspended in culture media, which largely depend upon the effective density and diameter of formed agglomerates in suspension. Here we present a rapid and inexpensive method for accurately measuring the effective density of nano-agglomerates in suspension. This novel method is based on the volume of the pellet obtained by benchtop centrifugation of nanomaterial suspensions in a packed cell volume tube, and is validated against gold-standard analytical ultracentrifugation data. This simple and cost-effective method allows nanotoxicologists to correctly model nanoparticle transport, and thus attain accurate dosimetry in cell culture systems, which will greatly advance the development of reliable and efficient methods for toxicological testing and investigation of nano-bio interactions in vitro.Entities:
Mesh：
Year: 2014 PMID： 24675174 PMCID： PMC4038248 DOI： 10.1038/ncomms4514
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919