Literature DB >> 24674076

Western blotting using chemiluminescent substrates.

Alice Alegria-Schaffer1.   

Abstract

Western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture (Towbin et al., 1979). The technique enables indirect detection of protein samples immobilized on a nitrocellulose or polyvinylidene fluoride (PVDF) membrane.
Copyright © 2014 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Horseradish peroxidase (HRP); Nitrocellulose membrane; Polyvinylidene fluoride (PVDF) membrane; Protein transfer; SDS-PAGE; Western blotting

Mesh：

Substances：

Year:  2014        PMID： 24674076     DOI： 10.1016/B978-0-12-420119-4.00019-7

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  7 in total

1.  A High-Throughput Drug Screening Strategy for Detecting Rhodopsin P23H Mutant Rescue and Degradation.

Authors:  Yuanyuan Chen; Hong Tang; William Seibel; Ruben Papoian; Xiaoyu Li; Nevin A Lambert; Krzysztof Palczewski
Journal:  Invest Ophthalmol Vis Sci       Date:  2015-04       Impact factor: 4.799

2.  Morbid obesity-related changes in the expression of lipid receptors, transporters, and HSL in human sperm.

Authors:  Berniza Calderón; Lydia Huerta; María Emilia Casado; José Manuel González-Casbas; José Ignacio Botella-Carretero; Antonia Martín-Hidalgo
Journal:  J Assist Reprod Genet       Date:  2019-01-18       Impact factor: 3.412

3.  A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions.

Authors:  Tabitha A Peterson; Mark A Stamnes; Robert C Piper
Journal:  J Vis Exp       Date:  2018-06-06       Impact factor: 1.355

4.  Melatonin stimulates dendrite formation and complexity in the hilar zone of the rat hippocampus: participation of the Ca++/Calmodulin complex.

Authors:  Aline Domínguez-Alonso; Marcela Valdés-Tovar; Héctor Solís-Chagoyán; Gloria Benítez-King
Journal:  Int J Mol Sci       Date:  2015-01-16       Impact factor: 5.923

5.  Improved secretory expression of lignocellulolytic enzymes in Kluyveromyces marxianus by promoter and signal sequence engineering.

Authors:  Jungang Zhou; Peixia Zhu; Xiaoyue Hu; Hong Lu; Yao Yu
Journal:  Biotechnol Biofuels       Date:  2018-08-29       Impact factor: 6.040

6.  Detection of Connexins in Liver Cells Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis.

Authors:  Joost Willebrords; Michaël Maes; Sara Crespo Yanguas; Bruno Cogliati; Mathieu Vinken
Journal:  Methods Mol Biol       Date:  2016

7.  Role of Nucleophosmin Gene Mutation in Leukemogenesis of Acute Myeloid Leukemia.

Authors:  Safaa A A Khaled; John Burthem; El-Badry E Abo Elnoor; Lobna F ElToni; Hanan M Ahmed; Sohier M Ahmed
Journal:  J Hematol       Date:  2017-01-10
  7 in total

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