Literature DB >> 24674069

One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE).

Julie L Brunelle1, Rachel Green2.   

Abstract

This protocol describes a denaturing polyacrylamide gel system utilizing sodium dodecyl sulfate (SDS) to separate protein molecules based on size as first described by Laemmli (1970). SDS-PAGE can be used to monitor protein purifications, check the purity of samples, and to estimate molecular weights for unknown proteins.
© 2014 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Ammonium persulfate (APS); One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE); Polyacrylamide; Polyacrylamide gel electrophoresis; SDS-PAGE gel; Stacking gel; Tetramethylethylenediamine (TEMED)

Mesh:

Substances:

Year:  2014        PMID: 24674069     DOI: 10.1016/B978-0-12-420119-4.00012-4

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN: 0076-6879            Impact factor:   1.600


  35 in total

1.  Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry.

Authors:  Claudia Siverino; Barbara Tabisz; Tessa Lühmann; Lorenz Meinel; Thomas Müller; Heike Walles; Joachim Nickel
Journal:  J Vis Exp       Date:  2018-03-29       Impact factor: 1.355

2.  Novel RNA-Binding Proteins Isolation by the RaPID Methodology.

Authors:  Nitzan Samra; Yoav Arava
Journal:  J Vis Exp       Date:  2016-09-30       Impact factor: 1.355

3.  Design of Heteronuclear Metalloenzymes.

Authors:  A Bhagi-Damodaran; P Hosseinzadeh; E Mirts; J Reed; I D Petrik; Y Lu
Journal:  Methods Enzymol       Date:  2016-07-26       Impact factor: 1.600

4.  Novel 5-Nitrofuran-Activating Reductase in Escherichia coli.

Authors:  Vuong Van Hung Le; Ieuan G Davies; Christina D Moon; David Wheeler; Patrick J Biggs; Jasna Rakonjac
Journal:  Antimicrob Agents Chemother       Date:  2019-10-22       Impact factor: 5.191

5.  Recombinant Inga Laurina Trypsin Inhibitor (ILTI) Production in Komagataella Phaffii Confirms Its Potential Anti-Biofilm Effect and Reveals an Anti-Tumoral Activity.

Authors:  Fábio C Carneiro; Simone S Weber; Osmar N Silva; Ana Cristina Jacobowski; Marcelo H S Ramada; Maria L R Macedo; Octávio L Franco; Nádia S Parachin
Journal:  Microorganisms       Date:  2018-04-28

6.  Suppression of µ1 subunit of the adaptor protein complex 2 reduces dengue virus release.

Authors:  Nopprarat Tongmuang; Umpa Yasamut; Sansanee Noisakran; Gopinathan Pillai Sreekanth; Pa-Thai Yenchitsomanus; Thawornchai Limjindaporn
Journal:  Virus Genes       Date:  2019-11-12       Impact factor: 2.332

7.  Identification of the interferon-inducible GTPase GBP1 as major restriction factor for the Hepatitis E virus.

Authors:  Mirco Glitscher; Kiyoshi Himmelsbach; Kathrin Woytinek; Anja Schollmeier; Reimar Johne; Gerrit J K Praefcke; Eberhard Hildt
Journal:  J Virol       Date:  2021-01-20       Impact factor: 5.103

8.  Proteomic analysis of a plastid gene encoding RPS4 mutant in Chinese cabbage (Brassica campestris L. ssp. pekinensis).

Authors:  Xiaoyan Tang; Fengyan Shi; Yiheng Wang; Shengnan Huang; Ying Zhao; Hui Feng
Journal:  Funct Integr Genomics       Date:  2021-12-08       Impact factor: 3.410

9.  Identification of RNA Binding Partners of CRISPR-Cas Proteins in Prokaryotes Using RIP-Seq.

Authors:  Sahil Sharma; Cynthia M Sharma
Journal:  Methods Mol Biol       Date:  2022

10.  Polymer Particles Bearing Recombinant LEL CD81 as Trapping Systems for Hepatitis C Virus.

Authors:  Dmitry Polyakov; Ekaterina Sinitsyna; Natalia Grudinina; Mariia Antipchik; Rodion Sakhabeev; Viktor Korzhikov-Vlakh; Mikhail Shavlovsky; Evgenia Korzhikova-Vlakh; Tatiana Tennikova
Journal:  Pharmaceutics       Date:  2021-05-07       Impact factor: 6.321

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.