| Literature DB >> 24674069 |
Julie L Brunelle1, Rachel Green2.
Abstract
This protocol describes a denaturing polyacrylamide gel system utilizing sodium dodecyl sulfate (SDS) to separate protein molecules based on size as first described by Laemmli (1970). SDS-PAGE can be used to monitor protein purifications, check the purity of samples, and to estimate molecular weights for unknown proteins.Entities:
Keywords: Ammonium persulfate (APS); One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE); Polyacrylamide; Polyacrylamide gel electrophoresis; SDS-PAGE gel; Stacking gel; Tetramethylethylenediamine (TEMED)
Mesh:
Substances:
Year: 2014 PMID: 24674069 DOI: 10.1016/B978-0-12-420119-4.00012-4
Source DB: PubMed Journal: Methods Enzymol ISSN: 0076-6879 Impact factor: 1.600