Literature DB >> 24674059

Coimmunoprecipitation of proteins from yeast.

Erica Gerace1, Danesh Moazed2.   

Abstract

This protocol outlines a procedure for testing whether two proteins interact. A target protein will be immunoprecipitated using an antibody that recognizes it (or a tagged version of the protein). The immunoprecipitated material will be separated by SDS-PAGE and analyzed by Western blotting to assess the presence of a candidate interacting protein(s).
© 2014 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Coimmunoprecipitation; Immunoprecipitations; Normalization of cell lysates; Protein–protein interactions; Schizosaccharomyces pombe; Sodium dodecyl sulfate polyacrylamide gel electrophoresis; Western blotting; Whole cell lysates

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Substances：

Year:  2014        PMID： 24674059      PMCID： PMC4370295          DOI： 10.1016/B978-0-12-420119-4.00002-1

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  2 in total

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Authors:  Erica L Gerace; Mario Halic; Danesh Moazed
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  2 in total
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6.  XRN1 Is a Species-Specific Virus Restriction Factor in Yeasts.

Authors:  Paul A Rowley; Brandon Ho; Sarah Bushong; Arlen Johnson; Sara L Sawyer
Journal:  PLoS Pathog       Date:  2016-10-06       Impact factor: 6.823

7.  Gibberellin and auxin signaling genes RGA1 and ARF8 repress accessory fruit initiation in diploid strawberry.

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  8 in total

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