| Literature DB >> 24674058 |
Abstract
Trichloroacetic acid (TCA) precipitation of proteins is commonly used to concentrate protein samples or remove contaminants, including salts and detergents, prior to downstream applications such as SDS-PAGE or 2D-gels. TCA precipitation denatures the protein, so it should not be used if the protein must remain in its folded state (e.g., if you want to measure a biochemical activity of the protein).Entities:
Keywords: Bromophenol blue; Deoxycholate-trichloroacetic acid precipitation; Dithiothreitol (DTT); Glycerol; Sodium dodecyl sulfate (SDS); Sodium hydroxide (NaOH); Trichloroacetic acid (TCA); Tris base
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Year: 2014 PMID： 24674058 DOI： 10.1016/B978-0-12-420119-4.00001-X
Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600