| Literature DB >> 2467181 |
Abstract
The number of rRNA genes in Mycobacterium smegmatis was examined by hybridization of BamHI and SalI digests of chromosomal DNA with 3'-end-labeled 5S, 16S, 23S rRNA and tRNA. Each RNA probe gave two hybridization bands. The PstI fragments of 6.6 kilobases were cloned to pBR322. The cloned DNA was characterized by restriction endonuclease mapping, DNA-RNA hybridization, and the R-loop technique.Entities:
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Year: 1988 PMID： 2467181 DOI： 10.1111/j.1348-0421.1988.tb01490.x
Source DB: PubMed Journal: Microbiol Immunol ISSN： 0385-5600 Impact factor: 1.955