Literature DB >> 2467165

K88 fimbrial antigens: identification of antigenic determinants by the use of synthetic peptides.

K A Krogfelt1, M Meldal, P Klemm.   

Abstract

Identification of antigenic determinants of K88 fimbriae was approached by immunization of rabbits with BSA conjugated synthetic peptides mimicking either a predicted common determinant or type specific determinants. Anti-peptide sera were assayed by ELISA and sandwich ELISA. It was shown that sera raised against the peptides corresponding to the K88ab and K88ad variants of the 213-219 segment were able to recognize the corresponding antigenic variant of the native fimbriae whereas the rest of the antisera did not to any significant degree react with intact fimbriae. In Western blots all anti-peptide sera were able to recognize the K88 proteins. Similarly in ELISA assays all raised sera showed affinity to denatured K88 proteins.

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Year:  1987        PMID: 2467165     DOI: 10.1016/0882-4010(87)90053-2

Source DB:  PubMed          Journal:  Microb Pathog        ISSN: 0882-4010            Impact factor:   3.738


  8 in total

1.  Purification and N-terminal sequence of the alpha subunit of antigen 43, a unique protein complex associated with the outer membrane of Escherichia coli.

Authors:  P Caffrey; P Owen
Journal:  J Bacteriol       Date:  1989-07       Impact factor: 3.490

2.  Epitope analysis of the F4 (K88) fimbrial antigen complex of enterotoxigenic Escherichia coli by using monoclonal antibodies.

Authors:  F G van Zijderveld; J Anakotta; R A Brouwers; A M van Zijderveld; D Bakker; F K de Graaf
Journal:  Infect Immun       Date:  1990-06       Impact factor: 3.441

3.  Identification of surface-exposed linear B-cell epitopes of the nonfimbrial adhesin CS31A of Escherichia coli by using overlapping peptides and antipeptide antibodies.

Authors:  M C Méchin; E Rousset; J P Girardeau
Journal:  Infect Immun       Date:  1996-09       Impact factor: 3.441

4.  Cloning of DNA sequences encoding foreign peptides and their expression in the K88 pili.

Authors:  G Thiry; A Clippe; T Scarcez; J Petre
Journal:  Appl Environ Microbiol       Date:  1989-04       Impact factor: 4.792

5.  Inhibition of adhesion of Escherichia coli k88ac fimbria to its receptor, intestinal mucin-type glycoproteins, by a monoclonal antibody directed against a variable domain of the fimbria.

Authors:  R Sun; T J Anderson; A K Erickson; E A Nelson; D H Francis
Journal:  Infect Immun       Date:  2000-06       Impact factor: 3.441

6.  Immunological studies of the disulfide bridge region of Pseudomonas aeruginosa PAK and PAO pilins, using anti-PAK pilus and antipeptide antibodies.

Authors:  K K Lee; P A Sastry; W Paranchych; R S Hodges
Journal:  Infect Immun       Date:  1989-02       Impact factor: 3.441

7.  Characterization of the binding specificity of K88ac and K88ad fimbriae of enterotoxigenic Escherichia coli by constructing K88ac/K88ad chimeric FaeG major subunits.

Authors:  Weiping Zhang; Ying Fang; David H Francis
Journal:  Infect Immun       Date:  2008-11-17       Impact factor: 3.441

Review 8.  Swine and cattle enterotoxigenic Escherichia coli-mediated diarrhea. Development of therapies based on inhibition of bacteria-host interactions.

Authors:  M Mouricout
Journal:  Eur J Epidemiol       Date:  1991-11       Impact factor: 8.082

  8 in total

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