Florian Stöckigt1, Sonja Pöhlmann2, Georg Nickenig2, Jörg Otto Schwab2, Jan Wilko Schrickel2. 1. Department of Medicine-Cardiology, University Hospital Bonn, Sigmund Freud Str. 25, 53127Bonn, Germany florian.stoeckigt@ukb.uni-bonn.de. 2. Department of Medicine-Cardiology, University Hospital Bonn, Sigmund Freud Str. 25, 53127Bonn, Germany.
Abstract
AIMS: Heart rate turbulence (HRT) is a prognostic parameter for risk stratification in patients suffering from coronary artery disease. The aims of this study were to demonstrate the feasibility of quantifying HRT in mice, both in long-term electrocardiograms (ECGs) as well as after extrastimulus pacing, and to analyse its characteristics. METHODS AND RESULTS: We performed long-term ECG recordings using implanted telemetric chips and electrophysiological (EP) investigations, using transvenously inserted EP catheters, in healthy mice. Heart rate turbulence was calculated using the established turbulence onset (TO) and slope (TS) algorithm. After spontaneous ventricular premature complexes (VPCs), we found a negative TO (-2.2 ± 7.5%) and positive TS (15.5 ± 18.3 ms/RR interval). Electrophysiological investigations revealed positive values for TO (0.6 ± 1.1%) and TS (6.5 ± 2.9 ms/RR interval) after extrastimulus pacing maneuvers. The shortening of the extrastimuli coupling intervals delivered during EP investigations significantly influenced TO (r = 0.57; P = 0.01): shorter coupling intervals provoked more positive TO values. CONCLUSION: Mice display both spontaneous and induced HRT. In terms of TO, VPCs generated by extrastimulus pacing are significantly dependent on the coupling interval. Determining HRT in mice is feasible and provides insight into basic mechanisms of blood pressure regulation, which is realized by the baroreflex. Published on behalf of the European Society of Cardiology. All rights reserved.
AIMS: Heart rate turbulence (HRT) is a prognostic parameter for risk stratification in patients suffering from coronary artery disease. The aims of this study were to demonstrate the feasibility of quantifying HRT in mice, both in long-term electrocardiograms (ECGs) as well as after extrastimulus pacing, and to analyse its characteristics. METHODS AND RESULTS: We performed long-term ECG recordings using implanted telemetric chips and electrophysiological (EP) investigations, using transvenously inserted EP catheters, in healthy mice. Heart rate turbulence was calculated using the established turbulence onset (TO) and slope (TS) algorithm. After spontaneous ventricular premature complexes (VPCs), we found a negative TO (-2.2 ± 7.5%) and positive TS (15.5 ± 18.3 ms/RR interval). Electrophysiological investigations revealed positive values for TO (0.6 ± 1.1%) and TS (6.5 ± 2.9 ms/RR interval) after extrastimulus pacing maneuvers. The shortening of the extrastimuli coupling intervals delivered during EP investigations significantly influenced TO (r = 0.57; P = 0.01): shorter coupling intervals provoked more positive TO values. CONCLUSION:Mice display both spontaneous and induced HRT. In terms of TO, VPCs generated by extrastimulus pacing are significantly dependent on the coupling interval. Determining HRT in mice is feasible and provides insight into basic mechanisms of blood pressure regulation, which is realized by the baroreflex. Published on behalf of the European Society of Cardiology. All rights reserved.
Authors: Julio P Dos Santos; Stephany B Lucina; Bruna N da Costa; Karla L C Olaguivel; Giovana L R Tuleski; Marlos G Sousa Journal: Open Vet J Date: 2021-11-13
Authors: Christian Jansen; Baravan Al-Kassou; Jennifer Lehmann; Alessandra Pohlmann; Johannes Chang; Michael Praktiknjo; Georg Nickenig; Christian P Strassburg; Jan W Schrickel; René Andrié; Markus Linhart; Jonel Trebicka Journal: PLoS One Date: 2018-04-10 Impact factor: 3.240