Rich Stoner1, Maggie L Chow1, Maureen P Boyle1, Ed S Lein1, Eric Courchesne1, Susan M Sunkin1, Peter R Mouton1, Subhojit Roy1, Anthony Wynshaw-Boris1, Sophia A Colamarino1. 1. University of California, San Diego, Autism Center of Excellence (R.S., M.L.C., M.P.B., E.C.), and the Departments of Neuroscience (R.S., M.L.C., M.P.B., S.R., E.C.) and Pathology (S.R.), University of California, San Diego, School of Medicine, La Jolla; Allen Institute for Brain Science, Seattle (M.P.B., S.M.S., E.S.L.); the Department of Pathology and Cell Biology, University of South Florida School of Medicine and Alzheimer's Institute and Research Center, Tampa (P.R.M.); the Department of Genetics and Genome Sciences, Case Western Reserve University School of Medicine, Cleveland (A.W.-B.); and the Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Palo Alto, CA (S.A.C.).
Abstract
BACKGROUND: Autism involves early brain overgrowth and dysfunction, which is most strongly evident in the prefrontal cortex. As assessed on pathological analysis, an excess of neurons in the prefrontal cortex among children with autism signals a disturbance in prenatal development and may be concomitant with abnormal cell type and laminar development. METHODS: To systematically examine neocortical architecture during the early years after the onset of autism, we used RNA in situ hybridization with a panel of layer- and cell-type-specific molecular markers to phenotype cortical microstructure. We assayed markers for neurons and glia, along with genes that have been implicated in the risk of autism, in prefrontal, temporal, and occipital neocortical tissue from postmortem samples obtained from children with autism and unaffected children between the ages of 2 and 15 years. RESULTS: We observed focal patches of abnormal laminar cytoarchitecture and cortical disorganization of neurons, but not glia, in prefrontal and temporal cortical tissue from 10 of 11 children with autism and from 1 of 11 unaffected children. We observed heterogeneity between cases with respect to cell types that were most abnormal in the patches and the layers that were most affected by the pathological features. No cortical layer was uniformly spared, with the clearest signs of abnormal expression in layers 4 and 5. Three-dimensional reconstruction of layer markers confirmed the focal geometry and size of patches. CONCLUSIONS: In this small, explorative study, we found focal disruption of cortical laminar architecture in the cortexes of a majority of young children with autism. Our data support a probable dysregulation of layer formation and layer-specific neuronal differentiation at prenatal developmental stages. (Funded by the Simons Foundation and others.).
BACKGROUND:Autism involves early brain overgrowth and dysfunction, which is most strongly evident in the prefrontal cortex. As assessed on pathological analysis, an excess of neurons in the prefrontal cortex among children with autism signals a disturbance in prenatal development and may be concomitant with abnormal cell type and laminar development. METHODS: To systematically examine neocortical architecture during the early years after the onset of autism, we used RNA in situ hybridization with a panel of layer- and cell-type-specific molecular markers to phenotype cortical microstructure. We assayed markers for neurons and glia, along with genes that have been implicated in the risk of autism, in prefrontal, temporal, and occipital neocortical tissue from postmortem samples obtained from children with autism and unaffected children between the ages of 2 and 15 years. RESULTS: We observed focal patches of abnormal laminar cytoarchitecture and cortical disorganization of neurons, but not glia, in prefrontal and temporal cortical tissue from 10 of 11 children with autism and from 1 of 11 unaffected children. We observed heterogeneity between cases with respect to cell types that were most abnormal in the patches and the layers that were most affected by the pathological features. No cortical layer was uniformly spared, with the clearest signs of abnormal expression in layers 4 and 5. Three-dimensional reconstruction of layer markers confirmed the focal geometry and size of patches. CONCLUSIONS: In this small, explorative study, we found focal disruption of cortical laminar architecture in the cortexes of a majority of young children with autism. Our data support a probable dysregulation of layer formation and layer-specific neuronal differentiation at prenatal developmental stages. (Funded by the Simons Foundation and others.).
Authors: Cynthia M Schumann; Cinnamon S Bloss; Cynthia Carter Barnes; Graham M Wideman; Ruth A Carper; Natacha Akshoomoff; Karen Pierce; Donald Hagler; Nicholas Schork; Catherine Lord; Eric Courchesne Journal: J Neurosci Date: 2010-03-24 Impact factor: 6.167
Authors: Eric Courchesne; Karen Pierce; Cynthia M Schumann; Elizabeth Redcay; Joseph A Buckwalter; Daniel P Kennedy; John Morgan Journal: Neuron Date: 2007-10-25 Impact factor: 17.173
Authors: C C Larsen; K Bonde Larsen; N Bogdanovic; H Laursen; N Graem; G Badsberg Samuelsen; B Pakkenberg Journal: Neuroscience Date: 2006-03-03 Impact factor: 3.590
Authors: Micaela Santos; Neha Uppal; Camilla Butti; Bridget Wicinski; James Schmeidler; Panteleimon Giannakopoulos; Helmut Heinsen; Christoph Schmitz; Patrick R Hof Journal: Brain Res Date: 2010-08-27 Impact factor: 3.252
Authors: Jerzy Wegiel; Izabela Kuchna; Krzysztof Nowicki; Humi Imaki; Jarek Wegiel; Elaine Marchi; Shuang Yong Ma; Abha Chauhan; Ved Chauhan; Teresa Wierzba Bobrowicz; Mony de Leon; Leslie A Saint Louis; Ira L Cohen; Eric London; W Ted Brown; Thomas Wisniewski Journal: Acta Neuropathol Date: 2010-03-03 Impact factor: 17.088
Authors: Christina Cordero; Gayle C Windham; Laura A Schieve; Margaret Daniele Fallin; Lisa A Croen; Anna Maria Siega-Riz; Stephanie M Engel; Amy H Herring; Alison M Stuebe; Catherine J Vladutiu; Julie L Daniels Journal: Autism Res Date: 2019-04-10 Impact factor: 5.216
Authors: Cheryl K Walker; Paula Krakowiak; Alice Baker; Robin L Hansen; Sally Ozonoff; Irva Hertz-Picciotto Journal: JAMA Pediatr Date: 2015-02 Impact factor: 16.193