| Literature DB >> 2466903 |
H Motojima1, T Kobayashi, M Shimane, S Kamachi, M Fukushima.
Abstract
Human granulocyte colony stimulating factor (G-CSF) was detected at the pM level by a simple sequential sandwich enzyme immunoassay. The polyclonal antiserum obtained by immunizing rabbits with recombinant G-CSF did not recognize other colony stimulating factors. The EIA developed gave satisfactory reproducibility as judged by an intra-assay precision of 4.3-6.2% and an interassay precision of 6.2-9.0%. Results from this assay procedure correlated well with those of a bioassay. The method could be performed within 6 h.Entities:
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Year: 1989 PMID: 2466903 DOI: 10.1016/0022-1759(89)90005-7
Source DB: PubMed Journal: J Immunol Methods ISSN: 0022-1759 Impact factor: 2.303