SCOPE: The long-term effect of exposure to relevant dietary levels of genistein (GEN) on estrogen receptor-positive (ER+) human breast cancer (MCF-7) progression after GEN withdrawal in athymic mice xenograft model was studied. MATERIALS AND METHODS: Feeding studies were conducted to determine the estrogenic effect of diets on MCF-7 tumor growth: (1) implantation (19 weeks) and withdrawal (6 weeks) of 17β-estradiol (E2 ); (2) dietary GEN 500 and 750 ppm during treatment/withdrawal for 23/10 and 15/9 weeks, respectively; and, (3) dietary soy protein isolate (SPI) containing GEN 180 ppm for 31/9 weeks of treatment/withdrawal. MCF-7 tumors grew fast in the presence of E2 implantation and abruptly regressed completely after E2 withdrawal. At different rates, dietary GEN alone (500 and 750 ppm) and GEN (180 ppm)-containing SPI stimulated MCF-7 tumor growth. After removal of the stimulus diet, tumors induced by 750 ppm GEN, but not 500 ppm GEN or SPI, regressed completely. The protein expression of epidermal growth factor receptor 2 (HER2) was higher in the GEN- and SPI-induced nonregressing (GINR) tumors compared to MCF-7 and E2 controls. CONCLUSION: Long-term consumption of low GEN doses (≤500 ppm) promotes MCF-7 tumor growth and results in GINR tumors with more aggressive and advanced growth phenotypes.
SCOPE: The long-term effect of exposure to relevant dietary levels of genistein (GEN) on estrogen receptor-positive (ER+) humanbreast cancer (MCF-7) progression after GEN withdrawal in athymic mice xenograft model was studied. MATERIALS AND METHODS: Feeding studies were conducted to determine the estrogenic effect of diets on MCF-7tumor growth: (1) implantation (19 weeks) and withdrawal (6 weeks) of 17β-estradiol (E2 ); (2) dietary GEN 500 and 750 ppm during treatment/withdrawal for 23/10 and 15/9 weeks, respectively; and, (3) dietary soy protein isolate (SPI) containing GEN 180 ppm for 31/9 weeks of treatment/withdrawal. MCF-7 tumors grew fast in the presence of E2 implantation and abruptly regressed completely after E2 withdrawal. At different rates, dietary GEN alone (500 and 750 ppm) and GEN (180 ppm)-containing SPI stimulated MCF-7tumor growth. After removal of the stimulus diet, tumors induced by 750 ppm GEN, but not 500 ppm GEN or SPI, regressed completely. The protein expression of epidermal growth factor receptor 2 (HER2) was higher in the GEN- and SPI-induced nonregressing (GINR) tumors compared to MCF-7 and E2 controls. CONCLUSION: Long-term consumption of low GEN doses (≤500 ppm) promotes MCF-7tumor growth and results in GINR tumors with more aggressive and advanced growth phenotypes.
Authors: Mengyuan Du; Xujuan Yang; James A Hartman; Paul S Cooke; Daniel R Doerge; Young H Ju; William G Helferich Journal: Carcinogenesis Date: 2012-01-20 Impact factor: 4.944
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Authors: G G Kuiper; J G Lemmen; B Carlsson; J C Corton; S H Safe; P T van der Saag; B van der Burg; J A Gustafsson Journal: Endocrinology Date: 1998-10 Impact factor: 4.736
Authors: Zeynep Madak-Erdogan; Ping Gong; Yiru Chen Zhao; Liwen Xu; Kinga U Wrobel; James A Hartman; Michelle Wang; Anthony Cam; Urszula T Iwaniec; Russell T Turner; Nathan C Twaddle; Daniel R Doerge; Ikhlas A Khan; John A Katzenellenbogen; Benita S Katzenellenbogen; William G Helferich Journal: Mol Nutr Food Res Date: 2015-11-10 Impact factor: 5.914