Literature DB >> 2466364

A rapid, quantitative assay for titration of bovine virus diarrhoea-mucosal disease virus.

P C Roberts1, J R Etchison, C W Bond.   

Abstract

An end point dilution microtitration assay is described that can be used for the titration of both cytopathic and non-cytopathic isolates of bovine virus diarrhoea-mucosal disease virus. Indirect immunofluorescence is used to detect infected MDBK cells in the wells of Terasaki plates. The virus titre is derived from the number of uninfected wells, using the Poisson distribution. The assay is simple, fast and economical. Titres of cytopathic virus determined by the microtitration assay and standard plaque assay are equivalent.

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Year:  1988        PMID： 2466364      PMCID： PMC7172778          DOI： 10.1016/0378-1135(88)90088-0

Source DB:  PubMed          Journal:  Vet Microbiol        ISSN： 0378-1135            Impact factor:   3.293


  17 in total

1.  Cellular resistance in tissue culture, induced by noncytopathogenic strains, to a cytopathogenic strain of virus diarrhea virus of cattle.

Authors:  J H GILLESPIE; S H MADIN; N B DARBY
Journal:  Proc Soc Exp Biol Med       Date:  1962-06

2.  Detection of bovine virus diarrhea virus in cell culture using an immunoperoxidase technique.

Authors:  J B Katz; L Ludemann; J Pemberton; M J Schmerr
Journal:  Vet Microbiol       Date:  1987-02       Impact factor: 3.293

Review 3.  Togaviridae.

Authors:  E G Westaway; M A Brinton; M C Horzinek; A Igarashi; L Kääriäinen; D K Lvov; J S Porterfield; P K Russell; D W Trent
Journal:  Intervirology       Date:  1985       Impact factor: 1.763

4.  Genetic analysis of simian virus 40. I. Description of microtitration and replica-plating techniques for virus.

Authors:  J A Robb; R G Martin
Journal:  Virology       Date:  1970-08       Impact factor: 3.616

5.  Plaque formation by non-cytopathogenic bovine virus diarrhea strains.

Authors:  P J Straver
Journal:  Arch Gesamte Virusforsch       Date:  1971

6.  Bovine diarrhea virus. I. Isolation of non-cytopathogenic strains detectable by END method.

Authors:  T Omori; Y Inaba; T Morimoto; Y Tanaka; H Kurogi
Journal:  Jpn J Microbiol       Date:  1967-06

7.  Reverse plaque formation method for titration of non-cytopathogenic bovine viral diarrhea-mucosal disease virus.

Authors:  O Itoh; H Sasaki; T Hanaki
Journal:  Natl Inst Anim Health Q (Tokyo)       Date:  1983

8.  Bovine viral diarrhea virus-infected MDBK monolayer as antigen in enzyme-linked immunosorbent assay (ELISA) for the measurement of antibodies in bovine sera.

Authors:  D M Justewicz; R Magar; G Marsolais; J Lecomte
Journal:  Vet Immunol Immunopathol       Date:  1987-04       Impact factor: 2.046

9.  Noncytopathogenic Bovine Viral Diarrhea Viruses Detected and Titrated by Immunofluorescence.

Authors:  A L Fernelius
Journal:  Can J Comp Med Vet Sci       Date:  1964-05

10.  Selected isolates of bovine viral diarrhea (BVD) virus propagated on bovine turbinate cells: virus titer and soluble antigen production as factors in immunogenicity of killed BVD virus.

Authors:  A W McClurkin; M F Coria
Journal:  Arch Virol       Date:  1978       Impact factor: 2.574
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  1 in total

1.  Comparative evaluation of the fluorescent antibody test and microtiter immunoperoxidase assay for detection of bovine viral diarrhea virus from bull semen.

Authors:  A Afshar; G C Dulac; C Dubuc; T H Howard
Journal:  Can J Vet Res       Date:  1991-01       Impact factor: 1.310
  1 in total

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