PURPOSE: To quantify the direct contribution of retinal ganglion cells (RGCs) on individual components of the mouse electroretinogram (ERG). METHODS: Dark- and light-adapted ERGs from mice 8 to 12 weeks after optic nerve transection (ONTx, n=14) were analyzed through stimulus response curves for a- and b-waves, oscillatory potentials (OPs), positive and negative scotopic threshold response (p/n STR), and the photopic negative response (PhNR) and compared with unoperated and sham-operated controls, as well as to eyes treated with 6-cyano-7-nitroquinoxaline-2,3-dion (CNQX). RESULTS: We confirmed in mice that CNQX intravitreal injection reduced the scotopic a-wave amplitude at high flash strength, confirming a post-receptoral contribution to the a-wave. We found that ONTx, which is more specific to RGCs, did not affect the a-wave amplitude and implicit time in either photopic or scotopic conditions while the b-wave was reduced. Both the pSTR and nSTR components were reduced in amplitude, with the balance between the two components resulting in a shortening of the nSTR peak implicit time. On the other hand, amplitude of the PhNR was increased while the OPs were minimally affected. CONCLUSION: With an intact a-wave demonstrated following ONTx, we find that the most robust indicators of RGC function in the mouse full-field ERG were the STR components.
PURPOSE: To quantify the direct contribution of retinal ganglion cells (RGCs) on individual components of the mouse electroretinogram (ERG). METHODS: Dark- and light-adapted ERGs from mice 8 to 12 weeks after optic nerve transection (ONTx, n=14) were analyzed through stimulus response curves for a- and b-waves, oscillatory potentials (OPs), positive and negative scotopic threshold response (p/n STR), and the photopic negative response (PhNR) and compared with unoperated and sham-operated controls, as well as to eyes treated with 6-cyano-7-nitroquinoxaline-2,3-dion (CNQX). RESULTS: We confirmed in mice that CNQX intravitreal injection reduced the scotopic a-wave amplitude at high flash strength, confirming a post-receptoral contribution to the a-wave. We found that ONTx, which is more specific to RGCs, did not affect the a-wave amplitude and implicit time in either photopic or scotopic conditions while the b-wave was reduced. Both the pSTR and nSTR components were reduced in amplitude, with the balance between the two components resulting in a shortening of the nSTR peak implicit time. On the other hand, amplitude of the PhNR was increased while the OPs were minimally affected. CONCLUSION: With an intact a-wave demonstrated following ONTx, we find that the most robust indicators of RGC function in the mouse full-field ERG were the STR components.
Authors: Garrett T Kenyon; Bryan J Travis; James Theiler; John S George; Gregory J Stephens; David W Marshak Journal: IEEE Trans Neural Netw Date: 2004-09
Authors: G B Jaissle; C A May; J Reinhard; K Kohler; S Fauser; E Lütjen-Drecoll; E Zrenner; M W Seeliger Journal: Invest Ophthalmol Vis Sci Date: 2001-02 Impact factor: 4.799
Authors: Nuwan Niyadurupola; Chi D Luu; Dan Q Nguyen; Kristen Geddes; Gary X V Tan; Celestine C W Wong; Tu Tran; Michael A Coote; Jonathan G Crowston Journal: Invest Ophthalmol Vis Sci Date: 2013-03-15 Impact factor: 4.799