BACKGROUND: The aim of this study was to investigate the distribution of full-length and deleted variants of KIR2DS4 in a population of southern Brazil and compare the results with other populations, as well as comparing two techniques, PCR-SSP and PCR-SSO, for typing of variants. METHODS: 258 individuals from southern Brazil were analysed by PCR-SSO ("polymerase chain reaction-sequence specific oligonucleotides", One Lambda, Inc., Canoga Park, CA), of which 161 were also analysed by PCR-SSP. RESULTS: The study population showed similarities with other Caucasian populations; 46.5% of individuals had only KIR2DS4 variants, 21.3% had the full-length form and 25.1% had both forms. CONCLUSION: The frequencies found in both groups (genotyped by PCR-SSP and PCR-SSO) were 100% concordant.
BACKGROUND: The aim of this study was to investigate the distribution of full-length and deleted variants of KIR2DS4 in a population of southern Brazil and compare the results with other populations, as well as comparing two techniques, PCR-SSP and PCR-SSO, for typing of variants. METHODS: 258 individuals from southern Brazil were analysed by PCR-SSO ("polymerase chain reaction-sequence specific oligonucleotides", One Lambda, Inc., Canoga Park, CA), of which 161 were also analysed by PCR-SSP. RESULTS: The study population showed similarities with other Caucasian populations; 46.5% of individuals had only KIR2DS4 variants, 21.3% had the full-length form and 25.1% had both forms. CONCLUSION: The frequencies found in both groups (genotyped by PCR-SSP and PCR-SSO) were 100% concordant.
Authors: W Luszczek; E Majorczyk; I Nowak; A Pawlik; M Jasek; A Wiśniewski; P Kuśnierczyk Journal: Int J Immunogenet Date: 2006-06 Impact factor: 1.466
Authors: M Jobim; P H Salim; P Portela; T J Wilson; J Fraportti; D Baronio; B Gil; L S Penna; R Roesler; L F Jobim; G Schwartsmann Journal: Int J Immunogenet Date: 2010-01-14 Impact factor: 1.466
Authors: Maureen P Martin; Xiaojiang Gao; Jeong-Hee Lee; George W Nelson; Roger Detels; James J Goedert; Susan Buchbinder; Keith Hoots; David Vlahov; John Trowsdale; Michael Wilson; Stephen J O'Brien; Mary Carrington Journal: Nat Genet Date: 2002-07-22 Impact factor: 38.330
Authors: Izabela Nowak; Maria Magott-Procelewska; Agnieszka Kowal; Maciej Miazga; Marta Wagner; Wanda Niepiekło-Miniewska; Małgorzata Kamińska; Andrzej Wiśniewski; Edyta Majorczyk; Marian Klinger; Wioleta Łuszczek; Andrzej Pawlik; Rafał Płoski; Ewa Barcz; David Senitzer; Piotr Kuśnierczyk Journal: PLoS One Date: 2012-09-13 Impact factor: 3.240