OBJECTIVES: The purpose of this study was to evaluate the effects of low-intensity pulsed ultrasound at 1.0 MHz on the healing process of fractures with bone loss in the rat fibula by alkaline phosphate level measurement and radiologic analyses. METHODS: Thirty 70-day-old male Wistar rats underwent a bone resection of 2.5 to 3.0 mm between the proximal and middle third of the right fibular diaphysis. The animals were randomly divided into 3 experimental groups: reference (uninjured), control (injured only), and treated (injured and treated with 5 applications of ultrasound, interspersed by 2 days of rest, beginning 24 hours after the osteotomy). Euthanasia was performed at experimental periods of 7 and 14 days. The right hind limb was removed for radiologic analysis. The blood was collected via cardiac puncture to determine the serum alkaline phosphatase activity. RESULTS: The bone fractures had not been completely consolidated in the treated and control group when analysis of the bone took place. At day 7, the serum alkaline phosphatase activity was higher in the treated group (mean ± SD, 72.17 ± 7.02 U/L) compared to the control (65.26 ± 8.41 U/L) and reference (67.21 ± 7.86 U/L) groups. At day 14, higher alkaline phosphatase activity was seen in the control group (68.96 ± 8.12 U/L) compared to the treated (66.09 ± 8.46 U/L) and reference (67.14 ± 7.96 U/L) groups. CONCLUSIONS: The biochemical and radiologic results suggest that low-intensity pulsed ultrasound can be used as an auxiliary method to consolidate fractures and probably reduces the bone healing time, offering clinical benefits.
OBJECTIVES: The purpose of this study was to evaluate the effects of low-intensity pulsed ultrasound at 1.0 MHz on the healing process of fractures with bone loss in the rat fibula by alkaline phosphate level measurement and radiologic analyses. METHODS: Thirty 70-day-old male Wistar rats underwent a bone resection of 2.5 to 3.0 mm between the proximal and middle third of the right fibular diaphysis. The animals were randomly divided into 3 experimental groups: reference (uninjured), control (injured only), and treated (injured and treated with 5 applications of ultrasound, interspersed by 2 days of rest, beginning 24 hours after the osteotomy). Euthanasia was performed at experimental periods of 7 and 14 days. The right hind limb was removed for radiologic analysis. The blood was collected via cardiac puncture to determine the serum alkaline phosphatase activity. RESULTS: The bone fractures had not been completely consolidated in the treated and control group when analysis of the bone took place. At day 7, the serum alkaline phosphatase activity was higher in the treated group (mean ± SD, 72.17 ± 7.02 U/L) compared to the control (65.26 ± 8.41 U/L) and reference (67.21 ± 7.86 U/L) groups. At day 14, higher alkaline phosphatase activity was seen in the control group (68.96 ± 8.12 U/L) compared to the treated (66.09 ± 8.46 U/L) and reference (67.14 ± 7.96 U/L) groups. CONCLUSIONS: The biochemical and radiologic results suggest that low-intensity pulsed ultrasound can be used as an auxiliary method to consolidate fractures and probably reduces the bone healing time, offering clinical benefits.
Authors: Renato de Moraes; Ana Maria de Guzzi Plepis; Virginia da Conceição Amaro Martins; Marco Antonio Hungaro Duarte; Murilo Priori Alcalde; Rogerio Leone Buchaim; Karina Torres Pomini; Eduardo Gomes Machado; Marcelo de Azevedo E Sousa Munhoz; Fernando Bento Cunha; Amanda Regina Alves Calegari; Amilton Iatecola; Samantha Ketelyn Silva; Marcelo Rodrigues da Cunha Journal: Am J Transl Res Date: 2019-08-15 Impact factor: 4.060
Authors: Marcelo de Azevedo E Sousa Munhoz; Karina Torres Pomini; Ana Maria de Guzzi Plepis; Virginia da Conceição Amaro Martins; Eduardo Gomes Machado; Renato de Moraes; Fernando Bento Cunha; Arnaldo Rodrigues Santos Junior; Guinea Brasil Camargo Cardoso; Marco Antonio Hungaro Duarte; Murilo Priori Alcalde; Daniela Vieira Buchaim; Rogerio Leone Buchaim; Marcelo Rodrigues da Cunha Journal: PLoS One Date: 2020-04-20 Impact factor: 3.240
Authors: Iris Jasmin Santos German; Karina Torres Pomini; Ana Carolina Cestari Bighetti; Jesus Carlos Andreo; Carlos Henrique Bertoni Reis; André Luis Shinohara; Geraldo Marco Rosa Júnior; Daniel de Bortoli Teixeira; Marcelie Priscila de Oliveira Rosso; Daniela Vieira Buchaim; Rogério Leone Buchaim Journal: Materials (Basel) Date: 2020-02-04 Impact factor: 3.623