Literature DB >> 2465397

Antigen preservation in microwave-irradiated tissues: a comparison with formaldehyde fixation.

A S Leong1, J Milios, C G Duncis.   

Abstract

A wide range of immunoreactive antigens commonly investigated in diagnostic pathology were evaluated in tissues subjected to fixation by microwave irradiation in normal saline to 63 degrees C. All of the 23 antigens studied were well preserved and compared with corresponding tissues conventionally fixed by formaldehyde for periods of 16 and 18 h, respectively, immunostaining in microwave-irradiated tissues was clearly superior, being more intense and also more extensive. Proteolytic digestion was not necessary for irradiated tissues except for the staining of cytokeratins and desmin. It was also possible to stain for Leu-14 (CD22) and Leu-1 (CD5), whereas these lymphocyte antigens were lost during formaldehyde fixation. Cytomorphological details were well preserved in the rapidly-fixed irradiated tissues.

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Year:  1988        PMID： 2465397     DOI： 10.1002/path.1711560402

Source DB:  PubMed          Journal:  J Pathol        ISSN： 0022-3417            Impact factor:   7.996


  10 in total

1.  A comparison of microwaves and heat alone in the preparation of tissue for electron microscopy.

Authors:  D Hopwood; G Milne; J Penston
Journal:  Histochem J       Date:  1990 Jun-Jul

2.  Cell growth and p53 expression in primary acquired melanosis and conjunctival melanoma.

Authors:  S Seregard
Journal:  J Clin Pathol       Date:  1996-04       Impact factor: 3.411

3.  Immunocytology on microwave-fixed cells reveals rapid and agonist-specific changes in subcellular accumulation patterns for cAMP or cGMP.

Authors:  J Barsony; S J Marx
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

4.  Comparison of routine fixation of tissues with rapid tissue fixation.

Authors:  Meenakshi Tripathi; Rani Bansal; Mamta Gupta; Vinay Bharat
Journal:  J Clin Diagn Res       Date:  2013-12-15

5.  Immediate fixation is cheaper than microwave fixation for obtaining good cytological details in frozen sections.

Authors:  J K Chan
Journal:  J Clin Pathol       Date:  1989-10       Impact factor: 3.411

6.  In situ characterization of the brain-microdevice interface using device-capture histology.

Authors:  Andrew J Woolley; Himanshi A Desai; Mitchell A Steckbeck; Neil K Patel; Kevin J Otto
Journal:  J Neurosci Methods       Date:  2011-07-23       Impact factor: 2.390

7.  Microwave processing of gustatory tissues for immunohistochemistry.

Authors:  Amanda Bond; John C Kinnamon
Journal:  J Neurosci Methods       Date:  2013-03-05       Impact factor: 2.390

8.  Immunohistochemical localization and quantification of the 3-(cystein-S-yl)-acetaminophen protein adduct in acetaminophen hepatotoxicity.

Authors:  D W Roberts; T J Bucci; R W Benson; A R Warbritton; T A McRae; N R Pumford; J A Hinson
Journal:  Am J Pathol       Date:  1991-02       Impact factor: 4.307

9.  Immunocytology with microwave-fixed fibroblasts shows 1 alpha,25-dihydroxyvitamin D3-dependent rapid and estrogen-dependent slow reorganization of vitamin D receptors.

Authors:  J Barsony; J W Pike; H F DeLuca; S J Marx
Journal:  J Cell Biol       Date:  1990-12       Impact factor: 10.539

Review 10.  Immunohistochemical diagnosis of human infectious diseases: a review.

Authors:  Hamadou Oumarou Hama; Gérard Aboudharam; Rémi Barbieri; Hubert Lepidi; Michel Drancourt
Journal:  Diagn Pathol       Date:  2022-01-30       Impact factor: 2.644
  10 in total

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