BACKGROUND: The rigorous cytological review by manual or automatic microscopic analysis is critical in the detection of circulating neoplastic cells, since their morphology as well as their count contributes to the diagnosis and prognosis of many diseases. However, the cytological analysis is not always obvious and requires trained and competent cytologist. In this context, the alarms and/or parameters generated by hematology analyzer could be particularly informative to alert the operators. METHODS: Blood samples from patients with Sezary syndrome (n = 9) were studied with Sysmex XN-1000 analyzer, and compared to patients with benign or tumoral skin lesions (n = 47) and patients with chronic lymphoproliferative B-cell diseases (n = 51) used as control. RESULTS: In present series, the value of structural lymphoid parameters (LyX and LyZ) and the alarm Blast/Abn Lympho were statistically higher in Sezary cases than in control cases. In addition, the value of LyX was associated to the count of circulating Sezary cells and value of LyZ to the presence of large Sezary cells, both parameters described as prognostic factors. CONCLUSION: The combination of alarm Blast/Abn Lympho and structural parameters (Ly-X/Ly-Z/Ly-Y) may allow to define rule of blood slide review to screen circulating Sezary cells, and give promising results in B-cell diseases.
BACKGROUND: The rigorous cytological review by manual or automatic microscopic analysis is critical in the detection of circulating neoplastic cells, since their morphology as well as their count contributes to the diagnosis and prognosis of many diseases. However, the cytological analysis is not always obvious and requires trained and competent cytologist. In this context, the alarms and/or parameters generated by hematology analyzer could be particularly informative to alert the operators. METHODS: Blood samples from patients with Sezary syndrome (n = 9) were studied with Sysmex XN-1000 analyzer, and compared to patients with benign or tumoral skin lesions (n = 47) and patients with chronic lymphoproliferative B-cell diseases (n = 51) used as control. RESULTS: In present series, the value of structural lymphoid parameters (LyX and LyZ) and the alarm Blast/Abn Lympho were statistically higher in Sezary cases than in control cases. In addition, the value of LyX was associated to the count of circulating Sezary cells and value of LyZ to the presence of large Sezary cells, both parameters described as prognostic factors. CONCLUSION: The combination of alarm Blast/Abn Lympho and structural parameters (Ly-X/Ly-Z/Ly-Y) may allow to define rule of blood slide review to screen circulating Sezary cells, and give promising results in B-cell diseases.
Authors: L Baseggio; F Berger; D Morel; M-H Delfau-Larue; G Goedert; G Salles; J-P Magaud; P Felman Journal: Leukemia Date: 2006-02 Impact factor: 11.528
Authors: I Theodorou; C Bigorgne; M H Delfau; C Lahet; G Cochet; M Vidaud; M Raphael; P Gaulard; J P Farcet Journal: J Pathol Date: 1996-03 Impact factor: 7.996
Authors: M G Bernengo; M Novelli; P Quaglino; F Lisa; A De Matteis; P Savoia; N Cappello; M T Fierro Journal: Br J Dermatol Date: 2001-01 Impact factor: 9.302
Authors: Sarah Birindelli; Maciej S Tarkowski; Marcello Gallucci; Marco Schiuma; Alice Covizzi; Przemysław Lewkowicz; Elena Aloisio; Felicia Stefania Falvella; Alberto Dolci; Agostino Riva; Massimo Galli; Mauro Panteghini Journal: Front Immunol Date: 2022-03-18 Impact factor: 7.561