Ling Yan Leung1, Cangel Pui Yee Chan1, Yuk Ki Leung1, Hui Lin Jiang2, Jill Morales Abrigo3, De Feng Wang3, Joseph Shun Hang Chung4, Timothy Hudson Rainer5, Colin Alexander Graham1. 1. Accident and Emergency Medicine Academic Unit, The Chinese University of Hong Kong, Hong Kong. 2. Department of Emergency, The Second Affiliated Hospital of Guangzhou Medical University, Guangdong, China. 3. Department of Imaging and Interventional Radiology, The Chinese University of Hong Kong, Hong Kong. 4. Accident and Emergency Department, Prince of Wales Hospital, Hong Kong. 5. Accident and Emergency Medicine Academic Unit, The Chinese University of Hong Kong, Hong Kong. Electronic address: thrainer@cuhk.edu.hk.
Abstract
BACKGROUND: The present study aimed to investigate and compare plasma concentrations of miR-124-3p and miR-16 as diagnostic markers in acute stroke. METHODS: miR-124-3p and miR-16 concentrations in 93 stroke patients were analyzed using real-time polymerase chain reaction. The primary outcome was the differentiation of hemorrhagic and ischemic stroke. RESULTS: Of 93 patients, 74 (79.6%) were diagnosed as ischemic stroke (IS) and 19 (20.4%) were diagnosed as hemorrhagic stroke (HS). Median plasma 124-3p concentrations taken within 24h of symptom onset were higher in HS patients than that in IS patients (3.8×10(5) vs 2.0×10(5) copies/ml plasma, p=0.0109), while median miR-16 concentration in IS patients were higher than that in HS patients (1.6×10(9) vs 1.3×10(9) copies/ml plasma, p=0.0399). The odds ratio (OR) for discriminating HS and IS with miR-124-3p and miR-16 was 5.37 and 9.75 respectively. CONCLUSION: Both miR-124-3p and miR-16 are diagnostic markers to discriminate HS and IS.
BACKGROUND: The present study aimed to investigate and compare plasma concentrations of miR-124-3p and miR-16 as diagnostic markers in acute stroke. METHODS:miR-124-3p and miR-16 concentrations in 93 strokepatients were analyzed using real-time polymerase chain reaction. The primary outcome was the differentiation of hemorrhagic and ischemic stroke. RESULTS: Of 93 patients, 74 (79.6%) were diagnosed as ischemic stroke (IS) and 19 (20.4%) were diagnosed as hemorrhagic stroke (HS). Median plasma 124-3p concentrations taken within 24h of symptom onset were higher in HSpatients than that in IS patients (3.8×10(5) vs 2.0×10(5) copies/ml plasma, p=0.0109), while median miR-16 concentration in IS patients were higher than that in HSpatients (1.6×10(9) vs 1.3×10(9) copies/ml plasma, p=0.0399). The odds ratio (OR) for discriminating HS and IS with miR-124-3p and miR-16 was 5.37 and 9.75 respectively. CONCLUSION: Both miR-124-3p and miR-16 are diagnostic markers to discriminate HS and IS.
Authors: Gang Lu; Man Sze Wong; Mark Zhi Qiang Xiong; Chi Kwan Leung; Xian Wei Su; Jing Ye Zhou; Wai Sang Poon; Vera Zhi Yuan Zheng; Wai Yee Chan; George Kwok Chu Wong Journal: J Am Heart Assoc Date: 2017-04-25 Impact factor: 5.501