Jing Zhang1, Yang Yang2, Yang Liu3, Yuxia Fan3, Zheng Liu3, Xiaoming Wang3, Qingling Yuan3, Yixing Yin3, Jie Yu3, Meiju Zhu3, Jian Zheng3, Xiubo Lu4. 1. Department of Thyroid Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China; Department of Pediatric Endocrinology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China. 2. Department of Thoracic surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China. 3. Department of Thyroid Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China. 4. Department of Thyroid Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China. Electronic address: luxiubo68877@126.com.
Abstract
BACKGROUND: Our recent study has found that microRNA-21 (miRNA-21) was significantly upregulated in papillary thyroid carcinoma (PTC) tissues compared with nontumor tissues by using miRNA microarray chip. However, the function of miRNA-21 is unknown in PTC. The aim of this study was to investigate the roles of miRNA-21 in PTC and the mechanism of gene regulation by it. METHODS: We transfected PTC cell line (TPC-1) with pEZX-eGFP-miRNA-21 plasmid to determine the biological functions of miRNA-21. Western blot assay was applied to investigate the correlation between miRNA-21 and programmed cell death 4 (PDCD4) expression in TPC-1 cell line. RESULTS: Overexpression of miRNA-21 could significantly enhance proliferation and invasion and inhibit the apoptosis of TPC-1 cells. In addition, miRNA-21 and PDCD4 expression showed a significantly negative correlation in TPC-1 cells. CONCLUSIONS: These data suggest that miRNA-21 may play an oncogenic role by directly targeting PDCD4 in the cellular processes of PTC. In addition, the findings in our present study also may represent new clues for the diagnostic and therapeutic strategies in the treatment of PTC.
BACKGROUND: Our recent study has found that microRNA-21 (miRNA-21) was significantly upregulated in papillary thyroid carcinoma (PTC) tissues compared with nontumor tissues by using miRNA microarray chip. However, the function of miRNA-21 is unknown in PTC. The aim of this study was to investigate the roles of miRNA-21 in PTC and the mechanism of gene regulation by it. METHODS: We transfected PTC cell line (TPC-1) with pEZX-eGFP-miRNA-21 plasmid to determine the biological functions of miRNA-21. Western blot assay was applied to investigate the correlation between miRNA-21 and programmed cell death 4 (PDCD4) expression in TPC-1 cell line. RESULTS: Overexpression of miRNA-21 could significantly enhance proliferation and invasion and inhibit the apoptosis of TPC-1 cells. In addition, miRNA-21 and PDCD4 expression showed a significantly negative correlation in TPC-1 cells. CONCLUSIONS: These data suggest that miRNA-21 may play an oncogenic role by directly targeting PDCD4 in the cellular processes of PTC. In addition, the findings in our present study also may represent new clues for the diagnostic and therapeutic strategies in the treatment of PTC.
Authors: Manuel Saiselet; Jaime M Pita; Alice Augenlicht; Geneviève Dom; Maxime Tarabichi; Danai Fimereli; Jacques E Dumont; Vincent Detours; Carine Maenhaut Journal: Oncotarget Date: 2016-08-09