Literature DB >> 2464706

Inhibition of RNase H activity and viral replication by single mutations in the 3' region of Moloney murine leukemia virus reverse transcriptase.

R Repaske1, J W Hartley, M F Kavlick, R R O'Neill, J B Austin.   

Abstract

Selected conserved amino acids in the putative RNase H domain of reverse transcriptase (RT) were modified in a molecularly cloned infectious provirus and in a Moloney murine leukemia virus RT expression vector by site-directed mutagenesis. Substitution of either of two conserved aspartic acid residues in proviral DNA prevented production of infectious particles in transfected NIH 3T3 cells, and the same modifications depressed RT-associated RNase H activity by more than 25-fold with little or no effect on polymerase activity.

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Year:  1989        PMID： 2464706      PMCID： PMC247852     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  38 in total

1.  Nucleotide sequence of the AIDS virus, LAV.

Authors:  S Wain-Hobson; P Sonigo; O Danos; S Cole; M Alizon
Journal:  Cell       Date:  1985-01       Impact factor: 41.582

2.  Sequence similarity among retroviruses--erratum.

Authors:  R Patarca; W A Haseltine
Journal:  Nature       Date:  1984 Jun 21-27       Impact factor: 49.962

3.  Involvement of retrovirus reverse transcriptase-associated RNase H in the initiation of strong-stop (+) DNA synthesis and the generation of the long terminal repeat.

Authors:  R Resnick; C A Omer; A J Faras
Journal:  J Virol       Date:  1984-09       Impact factor: 5.103

4.  Nucleotide sequence of Rous sarcoma virus.

Authors:  D E Schwartz; R Tizard; W Gilbert
Journal:  Cell       Date:  1983-03       Impact factor: 41.582

5.  Complete nucleotide sequence of the genome of bovine leukemia virus: its evolutionary relationship to other retroviruses.

Authors:  N Sagata; T Yasunaga; J Tsuzuku-Kawamura; K Ohishi; Y Ogawa; Y Ikawa
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

6.  Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template.

Authors:  M J Zoller; M Smith
Journal:  DNA       Date:  1984-12

7.  Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis B virus and cauliflower mosaic virus.

Authors:  H Toh; H Hayashida; T Miyata
Journal:  Nature       Date:  1983 Oct 27-Nov 2       Impact factor: 49.962

8.  Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA.

Authors:  M Seiki; S Hattori; Y Hirayama; M Yoshida
Journal:  Proc Natl Acad Sci U S A       Date:  1983-06       Impact factor: 11.205

9.  Murine leukemia virus mutant with a frameshift in the reverse transcriptase coding region: implications for pol gene structure.

Authors:  J G Levin; S C Hu; A Rein; L I Messer; B I Gerwin
Journal:  J Virol       Date:  1984-08       Impact factor: 5.103

10.  Construction and analysis of deletion mutations in the pol gene of Moloney murine leukemia virus: a new viral function required for productive infection.

Authors:  P Schwartzberg; J Colicelli; S P Goff
Journal:  Cell       Date:  1984-07       Impact factor: 41.582
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  26 in total

1.  Replication of phenotypically mixed human immunodeficiency virus type 1 virions containing catalytically active and catalytically inactive reverse transcriptase.

Authors:  J G Julias; A L Ferris; P L Boyer; S H Hughes
Journal:  J Virol       Date:  2001-07       Impact factor: 5.103

2.  The nature of the N-terminal amino acid residue of HIV-1 RNase H is critical for the stability of reverse transcriptase in viral particles.

Authors:  Guney Boso; Claes Örvell; Nikunj V Somia
Journal:  J Virol       Date:  2014-11-12       Impact factor: 5.103

3.  Mutations in the RNase H domain of HIV-1 reverse transcriptase affect the initiation of DNA synthesis and the specificity of RNase H cleavage in vivo.

Authors:  John G Julias; Mary Jane McWilliams; Stefan G Sarafianos; Edward Arnold; Stephen H Hughes
Journal:  Proc Natl Acad Sci U S A       Date:  2002-07-01       Impact factor: 11.205

4.  Defects in Moloney murine leukemia virus replication caused by a reverse transcriptase mutation modeled on the structure of Escherichia coli RNase H.

Authors:  A Telesnitsky; S W Blain; S P Goff
Journal:  J Virol       Date:  1992-02       Impact factor: 5.103

5.  Effects of mutations in the G tract of the human immunodeficiency virus type 1 polypurine tract on virus replication and RNase H cleavage.

Authors:  John G Julias; Mary Jane McWilliams; Stefan G Sarafianos; W Gregory Alvord; Eddy Arnold; Stephen H Hughes
Journal:  J Virol       Date:  2004-12       Impact factor: 5.103

6.  Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication.

Authors:  J C Guatelli; K M Whitfield; D Y Kwoh; K J Barringer; D D Richman; T R Gingeras
Journal:  Proc Natl Acad Sci U S A       Date:  1990-03       Impact factor: 11.205

7.  An unusual mechanism of self-primed reverse transcription requires the RNase H domain of reverse transcriptase to cleave an RNA duplex.

Authors:  H L Levin
Journal:  Mol Cell Biol       Date:  1996-10       Impact factor: 4.272

8.  Mutations in the 5' end of the human immunodeficiency virus type 1 polypurine tract affect RNase H cleavage specificity and virus titer.

Authors:  Mary Jane McWilliams; John G Julias; Stefan G Sarafianos; W Gregory Alvord; Edward Arnold; Stephen H Hughes
Journal:  J Virol       Date:  2003-10       Impact factor: 5.103

9.  Preferred sequences within a defined cleavage window specify DNA 3' end-directed cleavages by retroviral RNases H.

Authors:  Sharon J Schultz; Miaohua Zhang; James J Champoux
Journal:  J Biol Chem       Date:  2009-09-24       Impact factor: 5.157

10.  Mutation of amino acids in the connection domain of human immunodeficiency virus type 1 reverse transcriptase that contact the template-primer affects RNase H activity.

Authors:  John G Julias; Mary Jane McWilliams; Stefan G Sarafianos; W Gregory Alvord; Edward Arnold; Stephen H Hughes
Journal:  J Virol       Date:  2003-08       Impact factor: 5.103
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