Literature DB >> 2464563

A simple fixation and embedding method for use in hybridization histochemistry on plant tissues.

G I McFadden1, I Bonig, E C Cornish, A E Clarke.   

Abstract

A simple method for fixing and embedding plant tissue for hybridization histochemistry has been developed. Material is fixed with paraformaldehyde-glutaraldehyde by the phase-partition technique and embedded in LR Gold. The method preserves nucleic acids in situ. Double-stranded 32P-labelled DNA probes hybridized to complementary RNAs in thin and semithin sections. Biotinylated probes detected with immunogold markers have been used to localize transcripts at the electron microscopic level.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 2464563     DOI： 10.1007/bf01002612

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  25 in total

1.  In situ hybridization methods for the detection of somatostatin mRNA in tissue sections using antisense RNA probes.

Authors:  H Hoefler; H Childers; M R Montminy; R M Lechan; R H Goodman; H J Wolfe
Journal:  Histochem J       Date:  1986 Nov-Dec

2.  Efficiency of in situ hybridization as a function of probe size and fixation technique.

Authors:  T R Moench; H E Gendelman; J E Clements; O Narayan; D E Griffin
Journal:  J Virol Methods       Date:  1985-06       Impact factor: 2.014

Review 3.  Hybridization histochemistry.

Authors:  J D Penschow; J Haralambidis; P E Darling; I A Darby; E M Wintour; G W Tregear; J P Coghlan
Journal:  Experientia       Date:  1987-07-15

4.  Use of a biotinylated probe and in situ hybridization for light and electron microscopic localization of Po mRNA in myelin-forming Schwann cells.

Authors:  H F Webster; L Lamperth; J T Favilla; G Lemke; D Tesin; L Manuelidis
Journal:  Histochemistry       Date:  1987

5.  Quantitation in immunocytochemistry: correlation of immunogold labeling to absolute number of membrane antigens.

Authors:  G Griffiths; H Hoppeler
Journal:  J Histochem Cytochem       Date:  1986-11       Impact factor: 2.479

6.  Immunocytochemical localization of the cytochrome b/f complex of chloroplast thylakoid membranes.

Authors:  D J Goodchild; J M Anderson; B Andersson
Journal:  Cell Biol Int Rep       Date:  1985-08

7.  Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue.

Authors:  J Roth; M Bendayan; E Carlemalm; W Villiger; M Garavito
Journal:  J Histochem Cytochem       Date:  1981-05       Impact factor: 2.479

8.  The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions.

Authors:  E Kellenberger; M Dürrenberger; W Villiger; E Carlemalm; M Wurtz
Journal:  J Histochem Cytochem       Date:  1987-09       Impact factor: 2.479

9.  Dehydration and embedding temperatures affect the antigenic specificity of tubulin and immunolabeling by the protein A-colloidal gold technique.

Authors:  B L Armbruster; R M Garavito; E Kellenberger
Journal:  J Histochem Cytochem       Date:  1983-12       Impact factor: 2.479

10.  Expression sites and developmental regulation of genes encoding (1→3,1→4)-β-glucanases in germinated barley.

Authors:  G I McFadden; B Ahluwalia; A E Clarke; G B Fincher
Journal:  Planta       Date:  1988-12       Impact factor: 4.116
View more
  6 in total

1.  Patterns of soybean proline-rich protein gene expression.

Authors:  R E Wyatt; R T Nagao; J L Key
Journal:  Plant Cell       Date:  1992-01       Impact factor: 11.277

2.  Transformation and regeneration of the self-incompatible species Nicotiana alata Link & Otto.

Authors:  P R Ebert; A E Clarke
Journal:  Plant Mol Biol       Date:  1990-05       Impact factor: 4.076

Review 3.  Non-isotopic electron microscope in situ hybridization for studying the functional sub-compartmentalization of the cell nucleus.

Authors:  F Puvion-Dutilleul; E Puvion
Journal:  Histochem Cell Biol       Date:  1996-07       Impact factor: 4.304

4.  Polyembryony in Citrus. Accumulation of seed storage proteins in seeds and in embryos cultured in vitro.

Authors:  A M Koltunow; T Hidaka; S P Robinson
Journal:  Plant Physiol       Date:  1996-02       Impact factor: 8.340

5.  A molecular marker for lateral root initiation: the RSI-1 gene of tomato (Lycopersicon esculentum Mill) is activated in early lateral root primordia.

Authors:  B H Taylor; C F Scheuring
Journal:  Mol Gen Genet       Date:  1994-04

6.  An mRNA that specifically accumulates in maize roots delineates a novel subset of developing cortical cells.

Authors:  I John; H Wang; B M Held; E S Wurtele; J T Colbert
Journal:  Plant Mol Biol       Date:  1992-12       Impact factor: 4.076
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.