Literature DB >> 24643561

Virus-induced gene silencing and Agrobacterium tumefaciens-mediated transient expression in Nicotiana tabacum.

Zhao Zhang1, Bart P H J Thomma.   

Abstract

Virus-induced gene silencing (VIGS) is a rapid method for transient silencing of plant genes. In this chapter, we describe the methodology for Tobacco rattle virus (TRV)-based VIGS in Nicotiana tabacum. In combination with subsequent co-expression of the tomato immune receptor Ve1 and the corresponding Verticillium effector Ave1 through Agrobacterium tumefaciens-mediated transient transformation (agroinfiltration), we established a rapid system for assessing the requirement of candidate plant genes for Ve1-mediated immune signaling.

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Year:  2014        PMID： 24643561     DOI： 10.1007/978-1-62703-986-4_14

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  Verticillium Ave1 effector induces tomato defense gene expression independent of Ve1 protein.

Authors:  Christian Danve M Castroverde; Ross N Nazar; Jane Robb
Journal:  Plant Signal Behav       Date:  2016-11

2.  Comparative RNA-Seq analysis reveals a critical role for brassinosteroids in rose (Rosa hybrida) petal defense against Botrytis cinerea infection.

Authors:  Xintong Liu; Xiaoqian Cao; Shaochuan Shi; Na Zhao; Dandan Li; Peihong Fang; Xi Chen; Weicong Qi; Zhao Zhang
Journal:  BMC Genet       Date:  2018-08-20       Impact factor: 2.797

3.  RcTGA1 and glucosinolate biosynthesis pathway involvement in the defence of rose against the necrotrophic fungus Botrytis cinerea.

Authors:  Penghua Gao; Hao Zhang; Huijun Yan; Qigang Wang; Bo Yan; Hongying Jian; Kaixue Tang; Xianqin Qiu
Journal:  BMC Plant Biol       Date:  2021-05-17       Impact factor: 4.215

4.  Transcriptomic and metabolomic changes triggered by Macrosiphum rosivorum in rose (Rosa longicuspis).

Authors:  Penghua Gao; Hao Zhang; Huijun Yan; Ningning Zhou; Bo Yan; Yuanlan Fan; Kaixue Tang; Xianqin Qiu
Journal:  BMC Genomics       Date:  2021-12-09       Impact factor: 3.969

5.  Mutational analysis of the Ve1 immune receptor that mediates Verticillium resistance in tomato.

Authors:  Zhao Zhang; Yin Song; Chun-Ming Liu; Bart P H J Thomma
Journal:  PLoS One       Date:  2014-06-09       Impact factor: 3.240
  5 in total

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