| Literature DB >> 2464035 |
K Mohammad1, A Esen.
Abstract
The effect of blocking and non-blocking was compared in ELISA, immunostaining dot-blots and western blots using phosphate-buffered saline with and without the addition of Tween 20 for washes and dilutions. The results indicated that when Tween 20 was included in phosphate-buffered saline, a blocking agent and a blocking step were not required in immunoassays. Moreover, the use of protein-based blocking agents was found to somewhat lower the reactivity between the antibody and the antigen, possibly due to steric hindrance.Entities:
Mesh：
Substances：
Year: 1989 PMID： 2464035 DOI： 10.1016/0022-1759(89)90129-4
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303