| Literature DB >> 24639258 |
Serge Gambarelli1, Vincent Maurel.
Abstract
Electron paramagnetic resonance (EPR) is an invaluable tool when studying systems with paramagnetic centers. It is a sensitive spectroscopic method, which can be used with dilute samples in aqueous buffer solutions. Here, we describe the basic procedure for recording an X-band EPR spectrum of a metalloprotein sample at low temperature. We also discuss basic optimization techniques to provide spectra with a high signal to noise ratio and minimum distortion.Mesh：
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Year: 2014 PMID： 24639258 DOI： 10.1007/978-1-62703-794-5_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745