Literature DB >> 2463917

Conserved epitopes on plant H1 histones recognized by monoclonal antibodies.

L Mazzolini1, M Vaeck, M van Montagu.   

Abstract

A series of monoclonal antibodies specific for distinct regions of H1 histone from the plant Nicotiana tabacum were obtained from fusion experiments with spleen cells of mice immunized with tobacco nuclear extracts. These monoclonal antibodies were characterized and the evolutionary conservation of the epitopes in higher plants and animals studied by immunoblotting and enzyme-linked immunosorbent assay (ELISA). Whereas some epitopes appear restricted to the Solanaceae plant family, others are common to all higher eukaryotes tested and even detectable on nuclear proteins of yeast. ELISA experiments performed with isolated tobacco chromatin give some indications of the differential accessibility of the epitopes after interaction of H1 histone with the nucleosome.

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Year:  1989        PMID： 2463917     DOI： 10.1111/j.1432-1033.1989.tb14509.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  6 in total

1.  Production and characterisation of cell- and tissue-specific monoclonal antibodies for the flatworm Macrostomum sp.

Authors:  Peter Ladurner; Daniela Pfister; Christof Seifarth; Lukas Schärer; Monika Mahlknecht; Willi Salvenmoser; Regine Gerth; Florentine Marx; Reinhard Rieger
Journal:  Histochem Cell Biol       Date:  2004-11-18       Impact factor: 4.304

2.  Nucleotide sequence and expression of a maize H1 histone cDNA.

Authors:  P Razafimahatratra; N Chaubet; G Philipps; C Gigot
Journal:  Nucleic Acids Res       Date:  1991-04-11       Impact factor: 16.971

3.  Low levels of exogenous histone H1 in yeast cause cell death.

Authors:  G Miloshev; P Venkov; K van Holde; J Zlatanova
Journal:  Proc Natl Acad Sci U S A       Date:  1994-11-22       Impact factor: 11.205

4.  A bacterial peptide acting as a plant nuclear targeting signal: the amino-terminal portion of Agrobacterium VirD2 protein directs a beta-galactosidase fusion protein into tobacco nuclei.

Authors:  A Herrera-Estrella; M Van Montagu; K Wang
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

5.  Histone H1 overexpressed to high level in tobacco affects certain developmental programs but has limited effect on basal cellular functions.

Authors:  M Prymakowska-Bosak; M R Przewłoka; J Iwkiewicz; S Egierszdorff; M Kuraś; N Chaubet; C Gigot; S Spiker; A Jerzmanowski
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-17       Impact factor: 11.205

6.  Light induces rapid changes of the phosphorylation pattern in the cytosol of evacuolated parsley protoplasts.

Authors:  K Harter; H Frohnmeyer; S Kircher; T Kunkel; S Mühlbauer; E Schäfer
Journal:  Proc Natl Acad Sci U S A       Date:  1994-05-24       Impact factor: 11.205
  6 in total

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