Literature DB >> 2463835

Presence of RNA in the sperm nucleus.

C A Pessot1, M Brito, J Figueroa, I I Concha, A Yañez, L O Burzio.   

Abstract

The RNase-colloidal gold procedure for the ultrastructural localization of RNA was used for rat testis. Along with other structures, it was found that the testicular sperm nucleus was well stained. Similar labelling was observed in the nucleus of rat epididymal sperm and human sperm. The RNA was extracted from sperm and analyzed by electrophoresis on 10% polyacrylamide gel and 7 M urea. The electrophoretic profile revealed a complex set of bands ranging in size from tRNA to high molecular weight components. On the average, a content of about 0.1 pg of RNA per rat or human sperm was found.

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Year:  1989        PMID： 2463835     DOI： 10.1016/s0006-291x(89)80208-6

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  31 in total

1.  Cytochemical variations in the nucleolus during spermiogenesis in man and monkey.

Authors:  J P Dadoune; M F Alfonsi; M A Fain-Maurel
Journal:  Cell Tissue Res       Date:  1991-04       Impact factor: 5.249

2.  Sperm-borne miRNAs and endo-siRNAs are important for fertilization and preimplantation embryonic development.

Authors:  Shuiqiao Yuan; Andrew Schuster; Chong Tang; Tian Yu; Nicole Ortogero; Jianqiang Bao; Huili Zheng; Wei Yan
Journal:  Development       Date:  2015-12-30       Impact factor: 6.868

3.  Transcript expression profiles of Takifugu rubripes spermatozoa and eggs by expressed sequence tag analysis.

Authors:  Xue-Yan Shen; Jian-Zhou Cui; Qing-Li Gong; Yong-Jian Liu; Yoshitaka Nagahama
Journal:  Fish Physiol Biochem       Date:  2008-06-04       Impact factor: 2.794

4.  A cytochemical study of the nucleolus and nucleolus-related structures during human spermatogenesis.

Authors:  M Sousa; J Carvalheiro
Journal:  Anat Embryol (Berl)       Date:  1994-11

Review 5.  The presence, role and clinical use of spermatozoal RNAs.

Authors:  Meritxell Jodar; Sellappan Selvaraju; Edward Sendler; Michael P Diamond; Stephen A Krawetz
Journal:  Hum Reprod Update       Date:  2013-07-14       Impact factor: 15.610

6.  A novel SoxB2 gene is required for maturation of sperm nucleus during spermiogenesis in the Chinese mitten crab, Eriocheir sinensis.

Authors:  Zhi-Qiang Liu; Xue-Hui Jiang; Hai-Yan Qi; Liang-Wei Xiong; Gao-Feng Qiu
Journal:  Sci Rep       Date:  2016-08-26       Impact factor: 4.379

Review 7.  Chromosomal instability in mammalian pre-implantation embryos: potential causes, detection methods, and clinical consequences.

Authors:  Brittany L Daughtry; Shawn L Chavez
Journal:  Cell Tissue Res       Date:  2015-11-21       Impact factor: 5.249

8.  Evaluation of the effectiveness of semen storage and sperm purification methods for spermatozoa transcript profiling.

Authors:  Shihong Mao; Robert J Goodrich; Russ Hauser; Steven M Schrader; Zhen Chen; Stephen A Krawetz
Journal:  Syst Biol Reprod Med       Date:  2013-07-19       Impact factor: 3.061

Review 9.  Paternal contributions: new functional insights for spermatozoal RNA.

Authors:  Claudia Lalancette; David Miller; Yan Li; Stephen A Krawetz
Journal:  J Cell Biochem       Date:  2008-08-01       Impact factor: 4.429

Review 10.  Male Factors: the Role of Sperm in Preimplantation Embryo Quality.

Authors:  Zahra Bashiri; Fardin Amidi; Iraj Amiri; Zahra Zandieh; Chad B Maki; Fatemeh Mohammadi; Sadegh Amiri; Morteza Koruji
Journal:  Reprod Sci       Date:  2020-11-02       Impact factor: 3.060
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