Literature DB >> 24633791

Rapid measurement of mitotic spindle orientation in cultured mammalian cells.

Justin Decarreau1, Jonathan Driver, Charles Asbury, Linda Wordeman.   

Abstract

Factors that influence the orientation of the mitotic spindle are important for the maintenance of stem cell populations and in cancer development. However, screening for these factors requires rapid quantification of alterations of the angle of the mitotic spindle in cultured cell lines. Here we describe a method to image mitotic cells and rapidly score the angle of the mitotic spindle using a simple MATLAB application to analyze a stack of Z-images.

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Year:  2014        PMID： 24633791      PMCID： PMC4037854          DOI： 10.1007/978-1-4939-0329-0_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  External forces control mitotic spindle positioning.

Authors:  Jenny Fink; Nicolas Carpi; Timo Betz; Angelique Bétard; Meriem Chebah; Ammar Azioune; Michel Bornens; Cecile Sykes; Luc Fetler; Damien Cuvelier; Matthieu Piel
Journal:  Nat Cell Biol       Date:  2011-06-12       Impact factor: 28.824

2.  AMPK regulates mitotic spindle orientation through phosphorylation of myosin regulatory light chain.

Authors:  Jose T Thaiparambil; Carrie M Eggers; Adam I Marcus
Journal:  Mol Cell Biol       Date:  2012-06-11       Impact factor: 4.272

3.  Huntingtin is required for mitotic spindle orientation and mammalian neurogenesis.

Authors:  Juliette D Godin; Kelly Colombo; Maria Molina-Calavita; Guy Keryer; Diana Zala; Bénédicte C Charrin; Paula Dietrich; Marie-Laure Volvert; François Guillemot; Ioannis Dragatsis; Yohanns Bellaiche; Frédéric Saudou; Laurent Nguyen; Sandrine Humbert
Journal:  Neuron       Date:  2010-08-12       Impact factor: 17.173

4.  PtdIns(3,4,5)P3 regulates spindle orientation in adherent cells.

Authors:  Fumiko Toyoshima; Shigeru Matsumura; Hiroko Morimoto; Masaru Mitsushima; Eisuke Nishida
Journal:  Dev Cell       Date:  2007-12       Impact factor: 12.270

5.  Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation.

Authors:  Tomomi Kiyomitsu; Iain M Cheeseman
Journal:  Nat Cell Biol       Date:  2012-02-12       Impact factor: 28.824
  5 in total
  4 in total

1.  Automated classification and characterization of the mitotic spindle following knockdown of a mitosis-related protein.

Authors:  Matloob Khushi; Imraan M Dean; Erdahl T Teber; Megan Chircop; Jonathan W Arthur; Neftali Flores-Rodriguez
Journal:  BMC Bioinformatics       Date:  2017-12-28       Impact factor: 3.169

2.  Automated mitotic spindle tracking suggests a link between spindle dynamics, spindle orientation, and anaphase onset in epithelial cells.

Authors:  Matthew E Larson; William M Bement
Journal:  Mol Biol Cell       Date:  2017-01-18       Impact factor: 4.138

3.  The tetrameric kinesin Kif25 suppresses pre-mitotic centrosome separation to establish proper spindle orientation.

Authors:  Justin Decarreau; Michael Wagenbach; Eric Lynch; Aaron R Halpern; Joshua C Vaughan; Justin Kollman; Linda Wordeman
Journal:  Nat Cell Biol       Date:  2017-03-06       Impact factor: 28.824

4.  Automated tracking of S. pombe spindle elongation dynamics.

Authors:  Ana Sofía M Uzsoy; Parsa Zareiesfandabadi; Jamie Jennings; Alexander F Kemper; Mary Williard Elting
Journal:  J Microsc       Date:  2021-07-08       Impact factor: 1.952
  4 in total

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