| Literature DB >> 2463315 |
T Jitsukawa1, S Nakajima, I Sugawara, H Watanabe.
Abstract
Three monoclonal antibodies (MAbs) to recombinant interferon-gamma (rIFN-gamma) did not react well with rIFN-gamma immobilized on the ELISA plate surface. However, binding of the MAbs to immobilized rIFN-gamma was remarkably enhanced when a mixture of rIFN-gamma and bovine serum albumin (BSA) was used for coating. This enhancement was specific in terms of the antigen-antibody reaction. These results suggest that the amount of rIFN-gamma on the plate's plastic surface is increased and that the original structure of rIFN-gamma is preserved.Entities:
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Year: 1989 PMID： 2463315 DOI： 10.1016/0022-1759(89)90211-1
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303