Quanfu Zheng1, Yanling Zhao2, Jiabo Wang3, Tiantian Liu1, Bo Zhang4, Man Gong5, Jianyu Li5, Honghong Liu5, Bin Han6, Yaming Zhang3, Xueai Song5, Yonggang Li5, Xiaohe Xiao3. 1. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; China Military Institute of Chinese Medicine, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China. 2. China Military Institute of Chinese Medicine, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China. Electronic address: zhaoyanling2855@126.com. 3. China Military Institute of Chinese Medicine, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China. 4. The Second Hospital Affiliated to Harbin Medical University, No 86, Baojian Road, Nangang District, Harbin, Heilongjiang Province, 150086, China. Electronic address: zhangbo_6679@126.com. 5. Department of Integrative Medical Center, 302 Hospital of People׳s Liberation Army, 100, the 4th Ring Road, Beijing 100039, China. 6. Department of Pharmacy, Guang Dong Pharmacy College, 68, Nanhua District, Guangzhou 51006, China.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: The crude secondary roots of Aconitum carmichaelii Debeaux (Fuzi), together with its processed products, including Yanfuzi, Heishunpian and Paofupian, are commonly applied in clinic using for thousands of years, such as collapse, syncope, rheumatic fever, painful joints and various tumors. AIM OF THE STUDY: To explore the different effects of Fuzi and its processed products on energy metabolism, with mitochondria as the model with the aim of guiding the clinical use of Fuzi and its products. fingerprints of Fuzi, Yanfuzi, Heishunpian and Paofupian were established by Ultra-high Performance Liquid Chromatography (UPLC) and effects of Fuzi and its processed products on rat's liver׳s mitochondrial metabolism were studied by microcalorimetry. Spectrum-effect relationships between UPLC fingerprints and energy metabolism of mitochondria were investigated using canonical correlation analysis (CCA). RESULTS: Because of their inherent differences in chemical compositions, the main activities of energy metabolism of mitochondria were different among Fuzi and its processed products. The potential bioactivity sequence of the tested products was Fuzi>Heishunpian>Paofupian>Yanfuzi. RESULTS of CCA showed that compounds mesaconitine, benzoylaconitine, and benzoylhypacoitine might be the principal active components. CONCLUSION: Altogether, this work provides a general model of combination of UPLC and microcalorimetry to study the spectrum-effect relationships of Fuzi and its processed products which can offer some references for detecting principal components of traditional Chinese medicine on bioactivity to mitochondrial growth.
ETHNOPHARMACOLOGICAL RELEVANCE: The crude secondary roots of Aconitum carmichaelii Debeaux (Fuzi), together with its processed products, including Yanfuzi, Heishunpian and Paofupian, are commonly applied in clinic using for thousands of years, such as collapse, syncope, rheumatic fever, painful joints and various tumors. AIM OF THE STUDY: To explore the different effects of Fuzi and its processed products on energy metabolism, with mitochondria as the model with the aim of guiding the clinical use of Fuzi and its products. fingerprints of Fuzi, Yanfuzi, Heishunpian and Paofupian were established by Ultra-high Performance Liquid Chromatography (UPLC) and effects of Fuzi and its processed products on rat's liver׳s mitochondrial metabolism were studied by microcalorimetry. Spectrum-effect relationships between UPLC fingerprints and energy metabolism of mitochondria were investigated using canonical correlation analysis (CCA). RESULTS: Because of their inherent differences in chemical compositions, the main activities of energy metabolism of mitochondria were different among Fuzi and its processed products. The potential bioactivity sequence of the tested products was Fuzi>Heishunpian>Paofupian>Yanfuzi. RESULTS of CCA showed that compounds mesaconitine, benzoylaconitine, and benzoylhypacoitine might be the principal active components. CONCLUSION: Altogether, this work provides a general model of combination of UPLC and microcalorimetry to study the spectrum-effect relationships of Fuzi and its processed products which can offer some references for detecting principal components of traditional Chinese medicine on bioactivity to mitochondrial growth.