| Literature DB >> 24631548 |
Kun Wang1, Wei Li1, Xin Rui1, Xiaohong Chen1, Mei Jiang1, Mingsheng Dong2.
Abstract
Two released exopolysaccharides (r-EPS1 and r-EPS2) were isolated from the culture supernatant of Lactobacillus plantarum 70810 by ethanol precipitation, anion exchange and gel filtration chromatography and characterization of the structures were conducted. The r-EPS1 and r-EPS2 were homogenous polysaccharides with average molecular weights of, respectively, 204.6 and 202.8kDa, and composed of glucose, mannose and galactose with molar ratios of 18.21:78.76:3.03 and 12.92:30.89:56.19, respectively. Methylation and NMR analysis revealed r-EPS1 was consisted of alpha configuration sugar residues, whereas r-EPS2 contained both alpha and beta configuration sugar residues. Antioxidant and antitumor activities of the two exopolysaccharides were evaluated in vitro. The results showed that r-EPS1 and r-EPS2 exhibited potent antioxidant activities regarding hydroxyl and DPPH radicals scavenging and reducing power assays, and possessed antitumor activities against Caco-2, BGC-823 and HT-29 cells, which suggested that they could be potential sources of antioxidant and antitumor drug.Entities:
Keywords: Antitumor and antioxidant; Lactobacillus plantarum; Released exopolysaccharides (r-EPS)
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Year: 2014 PMID： 24631548 DOI： 10.1016/j.ijbiomac.2014.02.056
Source DB: PubMed Journal: Int J Biol Macromol ISSN： 0141-8130 Impact factor: 6.953