Literature DB >> 24626854

Lacking alignments? The next-generation sequencing mapper segemehl revisited.

Christian Otto1, Peter F Stadler2, Steve Hoffmann1.   

Abstract

MOTIVATION: Next-generation sequencing has become an important tool in molecular biology. Various protocols to investigate genomic, transcriptomic and epigenomic features across virtually all species and tissues have been devised. For most of these experiments, one of the first crucial steps of bioinformatic analysis is the mapping of reads to reference genomes.
RESULTS: Here, we present thorough benchmarks of our read aligner segemehl in comparison with other state-of-the-art methods. Furthermore, we introduce the tool lack to rescue unmapped RNA-seq reads which works in conjunction with segemehl and many other frequently used split-read aligners. AVAILABILITY: lack is distributed together with segemehl and freely available at www.bioinf.uni-leipzig.de/Software/segemehl/.
© The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

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Substances:

Year:  2014        PMID: 24626854     DOI: 10.1093/bioinformatics/btu146

Source DB:  PubMed          Journal:  Bioinformatics        ISSN: 1367-4803            Impact factor:   6.937


  33 in total

1.  Functional screenings reveal different requirements for host microRNAs in Salmonella and Shigella infection.

Authors:  Carmen Aguilar; Ana Rita Cruz; Ines Rodrigues Lopes; Claire Maudet; Ushasree Sunkavalli; Ricardo Jorge Silva; Malvika Sharan; Clivia Lisowski; Sara Zaldívar-López; Juan José Garrido; Mauro Giacca; Miguel Mano; Ana Eulalio
Journal:  Nat Microbiol       Date:  2019-12-02       Impact factor: 17.745

2.  Comparative Analysis Based on Transcriptomics and Metabolomics Data Reveal Differences between Emmer and Durum Wheat in Response to Nitrogen Starvation.

Authors:  Romina Beleggia; Nooshin Omranian; Yan Holtz; Tania Gioia; Fabio Fiorani; Franca M Nigro; Nicola Pecchioni; Pasquale De Vita; Ulrich Schurr; Jacques L David; Zoran Nikoloski; Roberto Papa
Journal:  Int J Mol Sci       Date:  2021-04-30       Impact factor: 5.923

3.  Analysis of a photosynthetic cyanobacterium rich in internal membrane systems via gradient profiling by sequencing (Grad-seq).

Authors:  Matthias Riediger; Philipp Spät; Raphael Bilger; Karsten Voigt; Boris Maček; Wolfgang R Hess
Journal:  Plant Cell       Date:  2021-04-17       Impact factor: 11.277

Review 4.  Emerging applications of read profiles towards the functional annotation of the genome.

Authors:  Sachin Pundhir; Panayiota Poirazi; Jan Gorodkin
Journal:  Front Genet       Date:  2015-05-19       Impact factor: 4.599

Review 5.  Bioinformatics of prokaryotic RNAs.

Authors:  Rolf Backofen; Fabian Amman; Fabrizio Costa; Sven Findeiß; Andreas S Richter; Peter F Stadler
Journal:  RNA Biol       Date:  2014-04-02       Impact factor: 4.652

6.  plantDARIO: web based quantitative and qualitative analysis of small RNA-seq data in plants.

Authors:  Deblina Patra; Mario Fasold; David Langenberger; Gerhard Steger; Ivo Grosse; Peter F Stadler
Journal:  Front Plant Sci       Date:  2014-12-23       Impact factor: 5.753

7.  The Mycobacterium tuberculosis transcriptional landscape under genotoxic stress.

Authors:  Amine Namouchi; Marta Gómez-Muñoz; Stephan A Frye; Line Victoria Moen; Torbjørn Rognes; Tone Tønjum; Seetha V Balasingham
Journal:  BMC Genomics       Date:  2016-10-10       Impact factor: 3.969

8.  Changes of the tRNA Modification Pattern during the Development of Dictyostelium discoideum.

Authors:  Anne Hoffmann; Lieselotte Erber; Heike Betat; Peter F Stadler; Mario Mörl; Jörg Fallmann
Journal:  Noncoding RNA       Date:  2021-05-28

9.  Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells.

Authors:  Carmen Aguilar; Susana Costa; Claire Maudet; R P Vivek-Ananth; Sara Zaldívar-López; Juan J Garrido; Areejit Samal; Miguel Mano; Ana Eulalio
Journal:  Nat Commun       Date:  2021-06-07       Impact factor: 14.919

10.  An investigation of causes of false positive single nucleotide polymorphisms using simulated reads from a small eukaryote genome.

Authors:  Antonio Ribeiro; Agnieszka Golicz; Christine Anne Hackett; Iain Milne; Gordon Stephen; David Marshall; Andrew J Flavell; Micha Bayer
Journal:  BMC Bioinformatics       Date:  2015-11-11       Impact factor: 3.169

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