Cervantes Caporossi1, Paulo Luiz Batista Nogueira2, José Carlos Costa Marques3, Rafael Moraes de Assis4, José Eduardo de Aguilar-Nascimento5. 1. Federal University of Mato Grosso, Department of Surgery, CuiabaMT, Brazil, PhD, Associate Professor, Division of Digestive Surgery, Department of Surgery, Federal University of Mato Grosso (UFMT), Cuiaba-MT, Brazil. Conception, design, intellectual and scientific content of the study. 2. UFMT, CuiabaMT, Brazil, Fellow Master degree, Postgraduate Program in Health Sciences, UFMT, Cuiaba-MT, Brazil. Acquisition and interpretation of data, manuscript writing. 3. University of Cuiaba, School of Medicine, Department of Surgery, CuiabaMT, Brazil, Assistant Professor, Surgical Techniques Division, Department of Surgery, School of Medicine, University of Cuiaba (UNIC), Cuiaba-MT, Brazil. Acquisition of data, statistical analysis. 4. UNIC, Faculty of Veterinary Medicine, CuiabaMT, Brazil, Graduate student, Faculty of Veterinary Medicine, UNIC, Cuiaba-MT, Brazil. Surgical procedures, acquisition of data. 5. UFMT, Department of Surgery, CuiabaMT, Brazil, PhD, Full Professor, Division of Digestive Surgery, Department of Surgery, UFMT, Cuiaba-MT, Brazil. Critical revision.
Abstract
PURPOSE: To validate the gastroschisis experimental model in female rats and the effects on the glutamine fetal morphology during pregnancy. METHODS: Twelve pregnant rats Wistar were separated in two groups: Group I (n = 6 rats, 71 fetuses) took glutamine and Group II (n = 6 rats, 75 fetuses) took isocaloric supplementation. At the 18th day of pregnancy, female rats were taken to hysterotomy and the fetuses which were selected for the act of gastroschisis were partially removed from the womb and by the laparotomy technique, the exclusion of the intestine was done. After that, fetuses were put in the womb cavity again and the rats' abdomen sutured. At the 21st day of pregnancy, date before delivery, by C-section ordinary animals and the ones with gastroschisis were removed and studied separately. The morphometrical parameters studied were the body weight (PC); the intestine weight (PI); the intestine length (CI) and its relations (PI/PC, PI/CI e PC-PI). RESULTS: The intestine weight (PI) and the intestine length (CI) were different in fetuses with gastroschisis (p<0.05), however no difference between the groups regarding supplementation with glutamine. CONCLUSIONS: The gastroschisis experimental model is valid and reproducible. The nutritional therapy with glutamine did not change the morphometrical parameters.
PURPOSE: To validate the gastroschisis experimental model in female rats and the effects on the glutamine fetal morphology during pregnancy. METHODS: Twelve pregnant rats Wistar were separated in two groups: Group I (n = 6 rats, 71 fetuses) took glutamine and Group II (n = 6 rats, 75 fetuses) took isocaloric supplementation. At the 18th day of pregnancy, female rats were taken to hysterotomy and the fetuses which were selected for the act of gastroschisis were partially removed from the womb and by the laparotomy technique, the exclusion of the intestine was done. After that, fetuses were put in the womb cavity again and the rats' abdomen sutured. At the 21st day of pregnancy, date before delivery, by C-section ordinary animals and the ones with gastroschisis were removed and studied separately. The morphometrical parameters studied were the body weight (PC); the intestine weight (PI); the intestine length (CI) and its relations (PI/PC, PI/CI e PC-PI). RESULTS: The intestine weight (PI) and the intestine length (CI) were different in fetuses with gastroschisis (p<0.05), however no difference between the groups regarding supplementation with glutamine. CONCLUSIONS: The gastroschisis experimental model is valid and reproducible. The nutritional therapy with glutamine did not change the morphometrical parameters.
Authors: Fieke Terstappen; Angela J C Tol; Hendrik Gremmels; Kimberley E Wever; Nina D Paauw; Jaap A Joles; Eline M van der Beek; A Titia Lely Journal: Nutrients Date: 2020-08-21 Impact factor: 5.717