Rodrigo de las Heras Kozma1, Edson Marcelino Alves2, Valter Abraão Barbosa-de-Oliveira1, Fernanda Degobbi Tenorio Quirino dos Santos Lopes3, Renan Cenize Guardia4, Henrique Vivi Buzo4, Carolina Arruda de Faria1, Camila Yamashita2, Manzelio Cavazzana Júnior5, Fernando Frei6, Maria José de Oliveira Ribeiro-Paes5, João Tadeu Ribeiro-Paes7. 1. University of São Paulo, São Paulo, Brazil, Graduate Student. University of São Paulo, São Paulo, Brazil. 2. Júlio de Mesquita Filho São Paulo State University, Assis School of Sciences and Languages, Assis, Brazil, Undergraduate Student. Júlio de Mesquita Filho São Paulo State University at Assis School of Sciences and Languages, Assis, Brazil. 3. Júlio de Mesquita Filho São Paulo State University, Botucatu, Brazil, Researcher. Júlio de Mesquita Filho São Paulo State University, Botucatu, Brazil. 4. Faculdades Integradas Padre Albino, Catanduva, Brazil, Undergraduate Student. Faculdades Integradas Padre Albino, Catanduva, Brazil. 5. Faculdades Integradas Padre Albino, Catanduva, Brazil, Associate Professor. Faculdades Integradas Padre Albino, Catanduva, Brazil. 6. Júlio de Mesquita Filho São Paulo State University, Assis School of Sciences and Languages, Assis, Brazil, Associate Professor. Júlio de Mesquita Filho São Paulo State University at Assis School of Sciences and Languages, Assis, Brazil. 7. Júlio de Mesquita Filho São Paulo State University, Assis School of Sciences and Languages, Assis, Brazil, Assistant Professor. Júlio de Mesquita Filho São Paulo State University at Assis School of Sciences and Languages, Assis, Brazil.
Abstract
OBJECTIVE: To describe a new murine model of cigarette smoke-induced emphysema. METHODS: Twenty-four male Wistar rats were divided into two groups: the cigarette smoke group, comprising 12 rats exposed to smoke from 12 commercial filter cigarettes three times a day (a total of 36 cigarettes per day) every day for 30 weeks; and the control group, comprising 12 rats exposed to room air three times a day every day for 30 weeks. Lung function was assessed by mechanical ventilation, and emphysema was morphometrically assessed by measurement of the mean linear intercept (Lm). RESULTS: The mean weight gain was significantly (approximately ten times) lower in the cigarette smoke group than in the control group. The Lm was 25.0% higher in the cigarette smoke group. There was a trend toward worsening of lung function parameters in the cigarette smoke group. CONCLUSIONS: The new murine model of cigarette smoke-induced emphysema and the methodology employed in the present study are effective and reproducible, representing a promising and economically viable option for use in studies investigating the pathophysiology of and therapeutic approaches to COPD.
OBJECTIVE: To describe a new murine model of cigarette smoke-induced emphysema. METHODS: Twenty-four male Wistar rats were divided into two groups: the cigarette smoke group, comprising 12 rats exposed to smoke from 12 commercial filter cigarettes three times a day (a total of 36 cigarettes per day) every day for 30 weeks; and the control group, comprising 12 rats exposed to room air three times a day every day for 30 weeks. Lung function was assessed by mechanical ventilation, and emphysema was morphometrically assessed by measurement of the mean linear intercept (Lm). RESULTS: The mean weight gain was significantly (approximately ten times) lower in the cigarette smoke group than in the control group. The Lm was 25.0% higher in the cigarette smoke group. There was a trend toward worsening of lung function parameters in the cigarette smoke group. CONCLUSIONS: The new murine model of cigarette smoke-induced emphysema and the methodology employed in the present study are effective and reproducible, representing a promising and economically viable option for use in studies investigating the pathophysiology of and therapeutic approaches to COPD.
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