| Literature DB >> 24625404 |
Ya Jie Sheng1, Sergei Eremin2, Tie Jun Mi1, Su Xia Zhang1, Jian Zhong Shen1, Zhan Hui Wang1.
Abstract
A fluorescence polarization immunoassay (FPIA) was developed for the analysis ofaflatoxins (AFs) using an anti-aflatoxin B1 (AFB1) monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed an IC50 value of 23.33 ng/mL with a limit of detection of 13.12 ng/mL for AFB1. The cross-reactivities of AFB1, AFB2, AFG1, AFG2, AFM1, and AFM2 with the antibody were 100%, 65.7%, 143%, 23.5%, 111.4%, and 2%, respectively. The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs, albeit not AFG2 and AFM2. The total time required for analyzing 96 samples in one microplate was less than 5 min. This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs.Entities:
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Year: 2014 PMID： 24625404 DOI： 10.3967/bes2014.027
Source DB: PubMed Journal: Biomed Environ Sci ISSN： 0895-3988 Impact factor: 3.118