Literature DB >> 24621229

Impact of transition from microscopy to molecular screening for detection of intestinal protozoa in Dutch patients.

S Svraka-Latifovic1, S Bouter, H Naus, L J Bakker, C P Timmerman, J W Dorigo-Zetsma.   

Abstract

Detection of intestinal protozoa by PCR methods has been described as being sensitive and specific, and as improving the diagnostic yield. Here we present the outcome of the transition from microscopy to molecular screening for detection of a select group of intestinal protozoa in faeces in our laboratory. Introduction of molecular screening for intestinal protozoa resulted in higher sensitivity, reduced hands-on-time, reduced time-to-results, leading to improved diagnostic efficiency.
© 2014 The Authors Clinical Microbiology and Infection © 2014 European Society of Clinical Microbiology and Infectious Diseases.

Entities:  

Keywords:  Diagnostic efficiency; PCR; microscopy; molecular detection; protozoa; triple faeces test

Mesh：

Year:  2014        PMID： 24621229     DOI： 10.1111/1469-0691.12623

Source DB:  PubMed          Journal:  Clin Microbiol Infect        ISSN： 1198-743X            Impact factor:   8.067


  7 in total

1.  Performance of Real-Time Polymerase Chain Reaction Assays for the Detection of 20 Gastrointestinal Parasites in Clinical Samples from Senegal.

Authors:  Doudou Sow; Philippe Parola; Khadime Sylla; Magatte Ndiaye; Pascal Delaunay; Philippe Halfon; Sabine Camiade; Thérèse Dieng; Roger C K Tine; Babacar Faye; Jean Louis Ndiaye; Yémou Dieng; Oumar Gaye; Didier Raoult; Fadi Bittar
Journal:  Am J Trop Med Hyg       Date:  2017-07       Impact factor: 2.345

2.  Multicenter evaluation of the BioFire FilmArray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis.

Authors:  Sarah N Buss; Amy Leber; Kimberle Chapin; Paul D Fey; Matthew J Bankowski; Matthew K Jones; Margarita Rogatcheva; Kristen J Kanack; Kevin M Bourzac
Journal:  J Clin Microbiol       Date:  2015-01-14       Impact factor: 5.948

3.  Utility of multiplex  polymerase chain reaction (PCR) in diarrhea-An Indian perspective.

Authors:  Balavinoth Ramakrishnan; Ram Gopalakrishnan; P Senthur Nambi; Suresh Kumar Durairajan; R Madhumitha; Anil Tarigopula; Chitra Chandran; V Ramasubramanian
Journal:  Indian J Gastroenterol       Date:  2018-09-22

4.  Etiologic and clinical characterization of community acquired gastroenteritis in adult patients in a Chilean emergency room by the FilmArray GI panel.

Authors:  Carlos Valenzuela; Paulette Legarraga; Arturo Peña; Alex Arenas; Loni Berkowitz; Gigliola Ramírez; Aniela Wozniak; Patricia García; Manuel Álvarez-Lobos
Journal:  PLoS One       Date:  2018-11-26       Impact factor: 3.240

5.  Molecular detection of Enteropathogens from diarrheic stool of HIV positive patients in Gondar, Ethiopia.

Authors:  Lubaba Seid; William Stokes; Abebe Genetu Bayih; Sisay Getie; Aberham Abere; Habtie Tesfa; Dylan R Pillai
Journal:  BMC Infect Dis       Date:  2018-07-31       Impact factor: 3.090

6.  The Novodiag® Stool parasites assay, an innovative high-plex technique for fast detection of protozoa, helminths and microsporidia in stool samples: a retrospective and prospective study.

Authors:  Sophie Hartuis; Rose-Anne Lavergne; Céline Nourrisson; Jaco Verweij; Guillaume Desoubeaux; Florian Lussac-Sorton; Jean-Philippe Lemoine; Estelle Cateau; Fakhri Jeddi; Philippe Poirier; Patrice Le Pape; Florent Morio
Journal:  Parasite       Date:  2022-05-13       Impact factor: 3.020

7.  Evaluation of a Novel Commercial Real-Time PCR Assay for the Simultaneous Detection of Cryptosporidium spp., Giardia duodenalis, and Entamoeba histolytica.

Authors:  Alejandro Dashti; Henar Alonso; Cristina Escolar-Miñana; Pamela C Köster; Begoña Bailo; David Carmena; David González-Barrio
Journal:  Microbiol Spectr       Date:  2022-05-03
  7 in total

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