| Literature DB >> 24619678 |
Roeland Lameris1, Famke L Schneiders, Tanja D de Gruijl, Hans J van der Vliet.
Abstract
Invariant natural killer T cells (iNKT) and dendritic cells (DC) play a central role in tumor immunity through downstream activation of immune effector cells by pro-inflammatory cytokines. Evidence is accumulating that the CD1d-iNKT cell axis can be effectively used to potentiate DC-based cancer vaccines. Here, we provide a detailed methodology for the generation of (CD1d-expressing) monocyte-derived DC (moDC) and their subsequent loading with the iNKT cell agonist α-galactosylceramide (α-GalCer) or their direct ligation by agonistic anti-CD1d monoclonal antibodies.Entities:
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Year: 2014 PMID： 24619678 DOI： 10.1007/978-1-4939-0345-0_14
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745