PURPOSE: To understand if repeated cycles (2-4 rounds) of gonadotropin stimulation could affect intracellular localization/content of proteins controlling cell cycle progression in mouse fallopian tubes (FT) and ovaries. METHODS: FT and ovaries of estrous mice (control) and of stimulated mice were analyzed to detect Oct-3/4, Sox-2, p53, β-catenin, pAKT and cyclin D1 localization/content. Spindles and chromosome alignment were analyzed in ovulated oocytes. RESULTS: After round 4, FT and ovaries of control and stimulated groups showed no differences in Oct-3/4, Sox-2 and β-catenin localization nor in Oct-3/4, Sox-2, p53, β-catenin and pAKT contents. Cyclin D1 level increased significantly in FT of treated mice. Oocytes number decreased meanwhile frequency of abnormal meiotic spindles increased with treatments. CONCLUSIONS: Repetitive stimulations affected oocyte spindle morphology but did not induce changes in a set of proteins involved in cell cycle progression, usually altered in ovarian cancer. The significant increase of cyclin D1 in the FT requires further investigation.
PURPOSE: To understand if repeated cycles (2-4 rounds) of gonadotropin stimulation could affect intracellular localization/content of proteins controlling cell cycle progression in mousefallopian tubes (FT) and ovaries. METHODS: FT and ovaries of estrousmice (control) and of stimulated mice were analyzed to detect Oct-3/4, Sox-2, p53, β-catenin, pAKT and cyclin D1 localization/content. Spindles and chromosome alignment were analyzed in ovulated oocytes. RESULTS: After round 4, FT and ovaries of control and stimulated groups showed no differences in Oct-3/4, Sox-2 and β-catenin localization nor in Oct-3/4, Sox-2, p53, β-catenin and pAKT contents. Cyclin D1 level increased significantly in FT of treated mice. Oocytes number decreased meanwhile frequency of abnormal meiotic spindles increased with treatments. CONCLUSIONS: Repetitive stimulations affected oocyte spindle morphology but did not induce changes in a set of proteins involved in cell cycle progression, usually altered in ovarian cancer. The significant increase of cyclin D1 in the FT requires further investigation.
Authors: Rebecca T Marquez; Keith A Baggerly; Andrea P Patterson; Jinsong Liu; Russell Broaddus; Michael Frumovitz; Edward N Atkinson; David I Smith; Lynn Hartmann; David Fishman; Andrew Berchuck; Regina Whitaker; David M Gershenson; Gordon B Mills; Robert C Bast; Karen H Lu Journal: Clin Cancer Res Date: 2005-09-01 Impact factor: 12.531
Authors: Ryo Matoba; Hitoshi Niwa; Shinji Masui; Satoshi Ohtsuka; Mark G Carter; Alexei A Sharov; Minoru S H Ko Journal: PLoS One Date: 2006-12-20 Impact factor: 3.240
Authors: Sevastiani Antonouli; Maria Grazia Palmerini; Serena Bianchi; Gianna Rossi; Sandra Cecconi; Manuel Belli; Sara Bernardi; Mohammad Ali Khalili; Giuseppe Familiari; Stefania Annarita Nottola; Guido Macchiarelli Journal: J Reprod Dev Date: 2020-04-28 Impact factor: 2.214