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Literature DB >> 2461704

A monoclonal antibody to aromatic amino acid hydroxylases. Identification of the epitope.

R G Cotton¹, W McAdam, I Jennings, F J Morgan.

Abstract

PH8 monoclonal antibody has previously been shown to react with all three aromatic amino acid hydroxylases, being particularly useful for immunohistochemical staining of brain tissue [Haan, Jennings, Cuello, Nakata, Chow, Kushinsky, Brittingham & Cotton (1987) Brain Res. 426, 19-27]. Western-blot analysis of liver extracts showed that PH8 reacted with phenylalanine hydroxylase from a wide range of vertebrate species. The epitope for antibody PH8 has been localized to the human phenylalanine hydroxylase sequence between amino acid residues 139 and 155. This highly conserved region of the aromatic amino acid hydroxylases has 11 out of 17 amino acids identical in phenylalanine hydroxylase, tyrosine hydroxylase and tryptophan hydroxylase.

Entities: Gene Species

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Year: 1988 PMID： 2461704 PMCID： PMC1135208 DOI： 10.1042/bj2550193

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

16 in total

1. Production of antibodies to rat liver phenylalanine hydroxylase. Cross-reactivity with other pterin-dependent hydroxylases.

Authors: P A Friedman; T Lloyd; S Kaufman
Journal: Mol Pharmacol Date: 1972-09 Impact factor: 4.436

2. Two apparent molecular weight forms of human and monkey phenylalanine hydroxylase are due to phosphorylation.

Authors: S C Smith; B E Kemp; W J McAdam; J F Mercer; R G Cotton
Journal: J Biol Chem Date: 1984-09-25 Impact factor: 5.157

3. Genetics of the mammalian phenylalanine hydroxylase system. II. Immunological and two-dimensional gel electrophoretic studies of phenylalanine hydroxylase in cultured normal and mutant rat hepatoma cells.

Authors: K H Choo; R G Cotton
Journal: Biochem Genet Date: 1979-10 Impact factor: 1.890

4. Comparative studies of four monoclonal antibodies to phenylalanine hydroxylase exhibiting different properties with respect to substrate-dependence, species-specificity and a range of effects on enzyme activity.

Authors: K H Choo; I G Jennings; R G Cotton
Journal: Biochem J Date: 1981-12-01 Impact factor: 3.857

5. A dot-immunobinding assay for monoclonal and other antibodies.

Authors: R Hawkes; E Niday; J Gordon
Journal: Anal Biochem Date: 1982-01-01 Impact factor: 3.365

6. Complete amino acid sequence of the goose-type lysozyme from the egg white of the black swan.

Authors: R J Simpson; G S Begg; D S Dorow; F J Morgan
Journal: Biochemistry Date: 1980-04-29 Impact factor: 3.162

7. Identification of two molecular-mass forms of phenylalanine hydroxylase that segregate independently in rats. Specific association of each form with certain rat strains.

Authors: J F Mercer; A Grimes; I Jennings; R G Cotton
Journal: Biochem J Date: 1984-05-01 Impact factor: 3.857

8. A monoclonal antibody to the phosphorylated form of phenylalanine hydroxylase. Definition of the phosphopeptide epitope.

Authors: S C Smith; W J McAdam; B E Kemp; F J Morgan; R G Cotton
Journal: Biochem J Date: 1987-06-15 Impact factor: 3.857

9. Distribution of phenylalanine hydroxylase (EC 1.14.3.1) in liver and kidney of vertebrates.

Authors: M C Hsieh; H K Berry
Journal: J Exp Zool Date: 1979-05

10. Serotonin-containing projections to the thalamus in the rat revealed by a horseradish peroxidase and peroxidase antiperoxidase double-staining technique.

Authors: A Consolazione; J V Priestley; A C Cuello
Journal: Brain Res Date: 1984-11-26 Impact factor: 3.252

15 in total

1. Pharmacology and distribution of norepinephrine transporters in the human locus coeruleus and raphe nuclei.

Authors: G A Ordway; C A Stockmeier; G W Cason; V Klimek
Journal: J Neurosci Date: 1997-03-01 Impact factor: 6.167

2. Tyrosine hydroxylase and tryptophan hydroxylase do not form heterotetramers.

Authors: S M Mockus; G J Yohrling; K E Vrana
Journal: J Mol Neurosci Date: 1998-02 Impact factor: 3.444

3. Differential involvement of hippocampal serotonin1A receptors and re-uptake sites in non-cognitive behaviors of Alzheimer's disease.

Authors: Mitchell K P Lai; Shirley W Tsang; Margaret M Esiri; Paul T Francis; Peter T-H Wong; Christopher P Chen
Journal: Psychopharmacology (Berl) Date: 2010-07-13 Impact factor: 4.530

Review 4. Structure and function of the aromatic amino acid hydroxylases.

Authors: S E Hufton; I G Jennings; R G Cotton
Journal: Biochem J Date: 1995-10-15 Impact factor: 3.857

5. Localization of cofactor binding sites with monoclonal anti-idiotype antibodies: phenylalanine hydroxylase.

Authors: I G Jennings; B E Kemp; R G Cotton
Journal: Proc Natl Acad Sci U S A Date: 1991-07-01 Impact factor: 11.205

6. Inputs to the ventrolateral bed nucleus of the stria terminalis.

Authors: Jung-Won Shin; Joel C Geerling; Arthur D Loewy
Journal: J Comp Neurol Date: 2008-12-10 Impact factor: 3.215

7. Melatonin Synthesis: Acetylserotonin O-Methyltransferase (ASMT) Is Strongly Expressed in a Subpopulation of Pinealocytes in the Male Rat Pineal Gland.

Authors: Martin F Rath; Steven L Coon; Fernanda G Amaral; Joan L Weller; Morten Møller; David C Klein
Journal: Endocrinology Date: 2016-03-07 Impact factor: 4.736