Epitope specificity and protective efficacy of the bovine immune response to bovine herpesvirus-1 glycoprotein vaccines.

Bovine herpesvirus-1 (BHV-1) envelope glycoproteins gI, gIII and gIV were individually purified on monoclonal antibody affinity columns and injected intradermally into BHV-1 seronegative calves. The calves developed serum neutralizing antibodies that monospecifically precipitated the immunizing glycoprotein from a preparation of 125I-labelled BHV-1 envelope proteins. A competitive radioimmunoassay using the bovine antisera demonstrated that known functional epitopes had been retained in the glycoprotein vaccines. Calves immunized with the gI, gIII or gIV glycoproteins were not protected from intranasal challenge with BHV-1 and had levels and duration of viral shedding in their nasal secretions similar to those of non-immunized control calves.