Literature DB >> 24609

A procedure for the assay of DNA damage in mammalian cells by alkaline elution and microfluorometric DNA determination.

G Brambilla, M Cavanna, L Sciabà, P Carlo, S Parodi, M Taningher.   

Abstract

The microfluorimetric assay of Kissane and Robins (J. Biol. Chem. 233, 184-188, 1958) has been modified to monitor DNA content in alkaline elution fractions. The elution profiles of DNA from treated and control cultured cells demonstrated a good correlation whether DNA in the elution fractions was determined by the fluorometric method or by measuring its radioactivity. The DNA recovery was of about 80% and the measured material was DNase digestible.

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Year:  1977        PMID： 24609

Source DB:  PubMed          Journal:  Ital J Biochem        ISSN： 0021-2938


  2 in total

1.  DNA damage and repair induced by diazoacetyl derivatives of amino acids with different mechanism of cytotoxicity. Correlations with mutagenicity and carcinogenicity.

Authors:  G Brambilla; M Cavanna; P Carlo; R Finollo; L Sciaba; S Parodi; C Bolognesi
Journal:  J Cancer Res Clin Oncol       Date:  1979-05-14       Impact factor: 4.553

2.  DNA fragmentation in some organs of rats and mice treated with cycasin.

Authors:  M Cavanna; S Parodi; M Taningher; C Bolognesi; L Sciabà; G Brambilla
Journal:  Br J Cancer       Date:  1979-04       Impact factor: 7.640
  2 in total

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