Literature DB >> 24607794

Dual genetically encoded phage-displayed ligands.

Kritika Mohan1, Gregory A Weiss2.   

Abstract

M13 bacteriophage display presents polypeptides as fusions to phage coat proteins. Such phage-displayed ligands offer useful reagents for biosensors. Here, we report a modified phage propagation protocol for the consistent and robust display of two different genetically encoded ligands on the major coat protein, P8. The results demonstrate that the phage surface reaches a saturation point for maximum peptide display.
Copyright © 2014 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Bacterial transformation; ELISA; Phage; Phage display

Mesh：

Substances：

Year:  2014        PMID： 24607794      PMCID： PMC4024456          DOI： 10.1016/j.ab.2014.02.025

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  11 in total

1.  Exploiting recombination in single bacteria to make large phage antibody libraries.

Authors:  D Sblattero; A Bradbury
Journal:  Nat Biotechnol       Date:  2000-01       Impact factor: 54.908

Review 2.  Engineering M13 for phage display.

Authors:  S S Sidhu
Journal:  Biomol Eng       Date:  2001-09

Review 3.  Filamentous phage display in the new millennium.

Authors:  John W Kehoe; Brian K Kay
Journal:  Chem Rev       Date:  2005-11       Impact factor: 60.622

Review 4.  Optimizing the affinity and specificity of proteins with molecular display.

Authors:  A M Levin; G A Weiss
Journal:  Mol Biosyst       Date:  2005-11-08

5.  Avoiding and controlling double transformation artifacts.

Authors:  Moshe Goldsmith; Csaba Kiss; Andrew R M Bradbury; Dan S Tawfik
Journal:  Protein Eng Des Sel       Date:  2007-06-15       Impact factor: 1.650

6.  Libraries of peptides and proteins displayed on filamentous phage.

Authors:  G P Smith; J K Scott
Journal:  Methods Enzymol       Date:  1993       Impact factor: 1.600

7.  Virus-polymer hybrid nanowires tailored to detect prostate-specific membrane antigen.

Authors:  Jessica A Arter; Juan E Diaz; Keith C Donavan; Tom Yuan; Reginald M Penner; Gregory A Weiss
Journal:  Anal Chem       Date:  2012-03-07       Impact factor: 6.986

8.  Sub-nanomolar detection of prostate-specific membrane antigen in synthetic urine by synergistic, dual-ligand phage.

Authors:  Kritika Mohan; Keith C Donavan; Jessica A Arter; Reginald M Penner; Gregory A Weiss
Journal:  J Am Chem Soc       Date:  2013-05-13       Impact factor: 15.419

9.  Phage wrapping with cationic polymers eliminates nonspecific binding between M13 phage and high pI target proteins.

Authors:  Jorge A Lamboy; Jessica A Arter; Kristeene A Knopp; Denise Der; Cathie M Overstreet; Edmund F Palermo; Hiromitsu Urakami; Ting-Bin Yu; Ozgul Tezgel; Gregory N Tew; Zhibin Guan; Kenichi Kuroda; Gregory A Weiss
Journal:  J Am Chem Soc       Date:  2009-11-18       Impact factor: 15.419

10.  Chemical and genetic wrappers for improved phage and RNA display.

Authors:  Jorge A Lamboy; Phillip Y Tam; Lucie S Lee; Pilgrim J Jackson; Sara K Avrantinis; Hye J Lee; Robert M Corn; Gregory A Weiss
Journal:  Chembiochem       Date:  2008-11-24       Impact factor: 3.164
View more
  3 in total

1.  Engineering chemically modified viruses for prostate cancer cell recognition.

Authors:  K Mohan; G A Weiss
Journal:  Mol Biosyst       Date:  2015-12

Review 2.  Chemically Modifying Viruses for Diverse Applications.

Authors:  Kritika Mohan; Gregory A Weiss
Journal:  ACS Chem Biol       Date:  2016-03-21       Impact factor: 5.100

3.  Biosensing with Virus Electrode Hybrids.

Authors:  Kritika Mohan; Reginald M Penner; Gregory A Weiss
Journal:  Curr Protoc Chem Biol       Date:  2015-06-01
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.