Grazielle Aparecida S Maia1, Vanessa Faria Cortes1, Rosy Iara Maciel de A Ribeiro2, Julio Alberto Mignaco3, Hérica de Lima Santos1, Carlos Frederico Leite Fontes4, Leandro A Barbosa5. 1. Laboratório de Bioquímica Celular, Faculdade de Bioquímica, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindu, São João del Rei, Brazil. 2. Laboratório de Biologia Celular e Mutagênese, Faculdade de Bioquímica, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindu, São João del Rei, Brazil. 3. Laboratório de Estrutura e Regulação de Proteínas e ATPases (LERPA), Programa de Biologia Estrutural, CCS, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. 4. Laboratório de Estrutura e Regulação de Proteínas e ATPases (LERPA), Programa de Biologia Estrutural, CCS, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: cfontes@bioqmed.ufrj.br. 5. Laboratório de Bioquímica Celular, Faculdade de Bioquímica, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindu, São João del Rei, Brazil. Electronic address: leaugust@yahoo.com.br.
Abstract
BACKGROUND: In developing countries, the access to red blood cell (RBC) irradiators is restricted. Thus, it is a common practice in blood banks to stock irradiated RBC units until they expire. The aim of this work is to elucidate the involvement of Na,K-ATPase in potassium leakage from prophylactically irradiated RBCs. METHODS: Whole blood was collected from healthy donors, and blood concentrates were irradiated with 25Gy of γ-radiation within 24h of collection. At days 3, 5, 7, 9, 11, 14 and 28 post-irradiation, fractions were removed and centrifuged and Na,K-ATPase activity from ghost membranes was determined. RESULTS: The inhibition of Na,K-ATPase activity in RBCs reached 12.6% by day 7 of storage and up to 50% by day 14 of storage. The addition of vitamin C prevented the irradiation-induced loss of Na,K-ATPase activity. The irradiation of RBCs provoked an increase in potassium plasma levels and a decrease in sodium plasma levels. The incubation of RBCs with ouabain did not change the sodium or potassium levels in the plasma, and the addition of vitamin C only partially prevented a decrease in sodium levels caused by irradiation. CONCLUSION: Because the inhibition of Na,K-ATPase by ouabain did not cause potassium accumulation in the plasma, we conclude that the irradiation-induced inhibition of the pump is not a key factor driving this effect.
BACKGROUND: In developing countries, the access to red blood cell (RBC) irradiators is restricted. Thus, it is a common practice in blood banks to stock irradiated RBC units until they expire. The aim of this work is to elucidate the involvement of Na,K-ATPase in potassium leakage from prophylactically irradiated RBCs. METHODS: Whole blood was collected from healthy donors, and blood concentrates were irradiated with 25Gy of γ-radiation within 24h of collection. At days 3, 5, 7, 9, 11, 14 and 28 post-irradiation, fractions were removed and centrifuged and Na,K-ATPase activity from ghost membranes was determined. RESULTS: The inhibition of Na,K-ATPase activity in RBCs reached 12.6% by day 7 of storage and up to 50% by day 14 of storage. The addition of vitamin C prevented the irradiation-induced loss of Na,K-ATPase activity. The irradiation of RBCs provoked an increase in potassium plasma levels and a decrease in sodium plasma levels. The incubation of RBCs with ouabain did not change the sodium or potassium levels in the plasma, and the addition of vitamin C only partially prevented a decrease in sodium levels caused by irradiation. CONCLUSION: Because the inhibition of Na,K-ATPase by ouabain did not cause potassium accumulation in the plasma, we conclude that the irradiation-induced inhibition of the pump is not a key factor driving this effect.
Authors: Jéssica Martins de Moura Valadares; Sumit O Bajaj; Hongyan Li; Hua-Yu L Wang; Simone Cavalcante Silva; Israel José Pereira Garcia; Duane Gischewiski Pereira; Pedro Azalim; Luis Eduardo Menezes Quintas; François Noël; Vanessa Faria Cortes; George Augustine O'Doherty; Leandro Augusto Barbosa Journal: J Cell Biochem Date: 2021-09-22 Impact factor: 4.429
Authors: Michał Bubiński; Agnieszka Gronowska; Paweł Szykuła; Agnieszka Woźniak; Aleksandra Rodacka; Scott Santi; Marcia Cardoso; Elżbieta Lachert Journal: Blood Transfus Date: 2022-02-01 Impact factor: 5.752