| Literature DB >> 24607168 |
Yee Huang1, Caihong Wu2, Zhenguang Liu1, Yuanliang Hu1, Cheng Shi1, Yun Yu1, Xiaojuan Zhao1, Cui Liu1, Jiaguo Liu1, Yi Wu1, Deyun Wang3.
Abstract
The aim of this study was to investigate the optimizing preparation conditions and immunological enhancement activity of Rehmannia glutinosa polysaccharide liposome (RGPL). RGPL was prepared using the reverse-phase evaporation method and optimized using the response surface methodology. The immunological enhancement activity of RGPL on splenocyte proliferation was measured. These results showed that the optimum preparation conditions were: a soybean phosphatide to cholesterol ratio of 8:1, a chloroform to methanol ratio of 3:5, a soybean phosphatide to tween 80 ratio of 10:1, a temperature (°C) of 66°C and an entrapment efficiency of 72.753 ± 0.318%. In a single stimulation of drugs, RGPL could significantly promote splenocyte proliferation, specifically at 200 μg mL(-1). Moreover, in a synergistic stimulation of drugs with LPS or PHA, a significant difference was obtained between the RGPL and RGP at 100-400 μg mL(-1), which indicated that the immunological enhancement of RGP was significantly improved after encapsulation with the liposome.Entities:
Keywords: Lymphocyte proliferation; Rehmannia glutinosa polysaccharide liposome; Response surface methodology
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Year: 2014 PMID： 24607168 DOI： 10.1016/j.carbpol.2014.01.022
Source DB: PubMed Journal: Carbohydr Polym ISSN： 0144-8617 Impact factor: 9.381