OBJECTIVE AND DESIGN: Mast cell (MC) degranulation can break peripheral immune tolerance. However, its mechanism remains unclear. Our goal was to study the stabilization of MC membranes by heme oxygenase-1 (HO-1) in order to influence dendritic cell (DC) function. MATERIAL: Mast cells and dendritic cells were prepared from 8-week-old to 10-week-old C57BL/6 mice; spleen mononuclear cells (SMCs) were prepared from 8-week-old to 10-week-old C57BL/6 and Balb/c mice. TREATMENT: Mast cells were pretreated with PBS, DMSO, Hemin (50 μl/ml), and Znpp (50 μl/ml) for 8 h. METHOD: Real-time PCR and western-blot tested the HO-1 of MC mRNA and protein. The co-stimulatory molecules of DCs (CD80, CD86, CD40) were measured by flow cytometry, and levels of TNF-α, IL-6, and IFN-γ were measured by ELISA. We set up a one-way mixed lymphocyte reaction (MLR) model to test the proliferation of SMCs after MC/DC interaction. *P < 0.05 (t test) was taken as the level of statistical significance. RESULT: MCs pretreated with hemin induced HO-1 mRNA and protein expression, then interacted with DCs; expression of the co-stimulatory molecules was attenuated. The TNF-α, IL-6, and IFN-γ levels in the co-culture system were decreased. These DCs couldn't stimulate the proliferation of SMCs. CONCLUSION: Inhibiting MC degranulation by HO-1 restrained DC maturation and attenuated the proliferation of SMCs.
OBJECTIVE AND DESIGN: Mast cell (MC) degranulation can break peripheral immune tolerance. However, its mechanism remains unclear. Our goal was to study the stabilization of MC membranes by heme oxygenase-1 (HO-1) in order to influence dendritic cell (DC) function. MATERIAL: Mast cells and dendritic cells were prepared from 8-week-old to 10-week-old C57BL/6 mice; spleen mononuclear cells (SMCs) were prepared from 8-week-old to 10-week-old C57BL/6 and Balb/c mice. TREATMENT: Mast cells were pretreated with PBS, DMSO, Hemin (50 μl/ml), and Znpp (50 μl/ml) for 8 h. METHOD: Real-time PCR and western-blot tested the HO-1 of MC mRNA and protein. The co-stimulatory molecules of DCs (CD80, CD86, CD40) were measured by flow cytometry, and levels of TNF-α, IL-6, and IFN-γ were measured by ELISA. We set up a one-way mixed lymphocyte reaction (MLR) model to test the proliferation of SMCs after MC/DC interaction. *P < 0.05 (t test) was taken as the level of statistical significance. RESULT: MCs pretreated with hemin induced HO-1 mRNA and protein expression, then interacted with DCs; expression of the co-stimulatory molecules was attenuated. The TNF-α, IL-6, and IFN-γ levels in the co-culture system were decreased. These DCs couldn't stimulate the proliferation of SMCs. CONCLUSION: Inhibiting MC degranulation by HO-1 restrained DC maturation and attenuated the proliferation of SMCs.
Authors: Kathrin Hochegger; Frank Siebenhaar; Volker Vielhauer; Dorothea Heininger; Tanya N Mayadas; Gert Mayer; Marcus Maurer; Alexander R Rosenkranz Journal: Eur J Immunol Date: 2005-10 Impact factor: 5.532
Authors: Li-Fan Lu; Evan F Lind; David C Gondek; Kathy A Bennett; Michael W Gleeson; Karina Pino-Lagos; Zachary A Scott; Anthony J Coyle; Jennifer L Reed; Jacques Van Snick; Terry B Strom; Xin Xiao Zheng; Randolph J Noelle Journal: Nature Date: 2006-08-20 Impact factor: 49.962
Authors: Sei-ichiro Tsuchihashi; Yuan Zhai; Qiao Bo; Ronald W Busuttil; Jerzy W Kupiec-Weglinski Journal: Transplantation Date: 2007-06-27 Impact factor: 4.939