Aldo Cunha Medeiros1, Ana Clara Batista Azevedo2, Jéssica Mayara de Figueiredo Oséas2, Mariana Davim Ferreira Gomes2, Fernanda Gurgel de Oliveira2, Keyla Borges Ferreira Rocha3, Italo Medeiros Azevedo4, Amália Cínthia Meneses Rêgo5, Marília Daniela Ferreira Carvalho5. 1. Federal University of Rio Grande do Norte, Department of Surgery, NatalRN, Brazil, Full Professor, Chairman, Department of Surgery, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil. Conception, design, intellectual and scientific content of the study; analysis and interpretation of data; technical procedures; critical revision. 2. UFRN, NatalRN, Brazil, Graduate student, Scientific Initiation Program, UFRN, Natal-RN, Brazil. Acquisition of data, manuscript preparation. 3. UFRN, Department of Pathology, NatalRN, Brazil, Associate Professor, Department of Pathology, UFRN, Natal-RN, Brazil. Acquisition and interpretation of data. 4. UFRN, Department of Surgery, NatalRN, Brazil, Master, Statistician, Department of Surgery, UFRN, Natal-RN, Brazil. Design of the study, interpretation of data, statistical analysis. 5. UFRN, NatalRN, Brazil, Fellow PhD degree, Postgraduate Program in Health Sciences, UFRN, Natal-RN, Brazil. Acquisition and interpretation of data.
Abstract
PURPOSE: To test the hypothesis that liver regeneration after partial hepatectomy can be influenced by the ileum. METHODS: Eighteen Wistar rats were distributed into groups of six animals: 1 - ileum resection+ hepatectomy 2/3; 2 - hepatectomy 2/3, and 3 - sham. Anesthesia with ketamine and xylazine i.p., aseptic technique, analgesia with meperidine (10mg/kg s.c.). On day 6, serum ALT, AST, alkaline phosphatase (AP) and albumin were measured. Liver regeneration and hepatocyte mitosis were quantified. Statistical analysis with ANOVA and Tukey tests, with significance p<0.05. RESULTS: In group hepatectomy+ileal resection, ALT, AST and AP were 180.6±24.9, 58.6±3.1 and 254.6±46.6 respectively. They were significantly higher than in the hepatectomy group, whose values were 126.0±16.5, 44.1±3.9 and 163.5±8.6, respectively (p<0.001). Albumin levels were not significantly different among groups. Liver regeneration in hepatectomy group (94.17%) was statistically higher (p<0.001) than in ileal resection+hepatectomy group (55.96%). In the latter group the mitosis of hepatocytes were significantly less frequent than in the hepatectomy group. CONCLUSION: The data confirm that the ileum positively influence on liver regeneration in rats undergoing hepatectomy.
PURPOSE: To test the hypothesis that liver regeneration after partial hepatectomy can be influenced by the ileum. METHODS: Eighteen Wistar rats were distributed into groups of six animals: 1 - ileum resection+ hepatectomy 2/3; 2 - hepatectomy 2/3, and 3 - sham. Anesthesia with ketamine and xylazine i.p., aseptic technique, analgesia with meperidine (10mg/kg s.c.). On day 6, serum ALT, AST, alkaline phosphatase (AP) and albumin were measured. Liver regeneration and hepatocyte mitosis were quantified. Statistical analysis with ANOVA and Tukey tests, with significance p<0.05. RESULTS: In group hepatectomy+ileal resection, ALT, AST and AP were 180.6±24.9, 58.6±3.1 and 254.6±46.6 respectively. They were significantly higher than in the hepatectomy group, whose values were 126.0±16.5, 44.1±3.9 and 163.5±8.6, respectively (p<0.001). Albumin levels were not significantly different among groups. Liver regeneration in hepatectomy group (94.17%) was statistically higher (p<0.001) than in ileal resection+hepatectomy group (55.96%). In the latter group the mitosis of hepatocytes were significantly less frequent than in the hepatectomy group. CONCLUSION: The data confirm that the ileum positively influence on liver regeneration in rats undergoing hepatectomy.