| Literature DB >> 24601828 |
Rishikesh Kumar1, Ganesh Chandra Sahoo, Krishna Pandey, Vnr Das, Pradeep Das.
Abstract
Drug delivery systems are a promising technology to increase poor solubility and bioavailability of compounds. Therefore we have developed PLGA-PEG encapsulated amphotericin B nanoparticles (NPs) drug delivery technology to increase the solubility of amphotericin B and target the macrophage of infected tissues during visceral leishmaniasis. The structural characterization by transmission electron microscopy and dynamic light scattering revealed the nano-size of the particle (30-35 nanometers). Fourier transform infrared spectroscopy confirmed the PLGA-PEG encapsulation. The mean cytotoxic assay (0.0803 + 0.0253) of extracellular promastigote of PLGA-PEG encapsulated amphotericin B is significantly lower than that of amphotericin B (0.1134 + 0.0153) and inhibition of amastigotes in the splenic tissue was significantly more than with conventional amphotericin B (93.02 + 6.63 versus 74.42 +14.78). Amphotericin B encapsulated PLGA-PEG nanoparticles were found to be more effective than free amphotericin B in terms of therapeutic efficacy during in vitro and in vivo study.Entities:
Keywords: Amastigotes; PLGA-PEG; amphotericin B; electron microscope; kala azar; nanometer; promastigote
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Year: 2014 PMID： 24601828 DOI： 10.3109/10717544.2014.891271
Source DB: PubMed Journal: Drug Deliv ISSN： 1071-7544 Impact factor: 6.419