BACKGROUND: WNT1 and WNT3A drive a dorsal to ventral gradient of β-catenin-dependent Wnt signaling in the developing spinal cord. However, the identity of the receptors mediating downstream functions remains poorly understood. RESULTS: In this report, we show that the spatiotemporal expression patterns of FZD10 and WNT1/WNT3A are highly correlated. We further show that in the presence of LRP6, FZD10 promotes WNT1 and WNT3A signaling using an 8xSuperTopFlash reporter assay. Consistent with a functional role for FZD10, we demonstrate that FZD10 is required for proliferation in the spinal cord. Finally, by using an in situ proximity ligation assay, we observe an interaction between FZD10 and WNT1 and WNT3A proteins. CONCLUSIONS: Together, our results identify FZD10 as a receptor for WNT1 and WNT3A in the developing chick spinal cord.
BACKGROUND:WNT1 and WNT3A drive a dorsal to ventral gradient of β-catenin-dependent Wnt signaling in the developing spinal cord. However, the identity of the receptors mediating downstream functions remains poorly understood. RESULTS: In this report, we show that the spatiotemporal expression patterns of FZD10 and WNT1/WNT3A are highly correlated. We further show that in the presence of LRP6, FZD10 promotes WNT1 and WNT3A signaling using an 8xSuperTopFlash reporter assay. Consistent with a functional role for FZD10, we demonstrate that FZD10 is required for proliferation in the spinal cord. Finally, by using an in situ proximity ligation assay, we observe an interaction between FZD10 and WNT1 and WNT3A proteins. CONCLUSIONS: Together, our results identify FZD10 as a receptor for WNT1 and WNT3A in the developing chick spinal cord.
Authors: Jan M Stenman; Jay Rajagopal; Thomas J Carroll; Makoto Ishibashi; Jill McMahon; Andrew P McMahon Journal: Science Date: 2008-11-21 Impact factor: 47.728
Authors: Vitezslav Bryja; Emma R Andersson; Alexandra Schambony; Milan Esner; Lenka Bryjová; Kristin K Biris; Anita C Hall; Bianca Kraft; Lukas Cajanek; Terry P Yamaguchi; Margaret Buckingham; Ernest Arenas Journal: Mol Biol Cell Date: 2008-12-03 Impact factor: 4.138
Authors: Carla Garcia-Morales; Chiung-Hui Liu; Muhammad Abu-Elmagd; Mohammad K Hajihosseini; Grant N Wheeler Journal: Dev Biol Date: 2009-08-28 Impact factor: 3.582
Authors: Emma R Andersson; Lenka Bryjova; Kristin Biris; Terry P Yamaguchi; Ernest Arenas; Vítezslav Bryja Journal: Dev Dyn Date: 2010-01 Impact factor: 3.780