Gi Jung Im1, Moo Kyun Park2, Jae-Jun Song3. 1. Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, South Korea. 2. Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul, South Korea. 3. Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, South Korea. Electronic address: jjsong23@gmail.com.
Abstract
OBJECTIVES: The aim of this study was to examine the cytotoxic effect and inflammatory response of human middle ear epithelial cells (HMEECs) induced by urban particles (UP). MATERIALS AND METHODS: Cell viability following UP exposure was assessed in HMEECs using the CCK 8 assay. The expression levels of the inflammation-related genes (COX-2 and MUC5AC) were analyzed using semi-quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and COX-2 production was analyzed using western blotting. RESULTS: Treatment with UP decreased cell viability in HMEECs in a dose- and time-dependent manner. UP exposure induced the significantly increased expression of COX-2 and MUC5AC. Western blotting showed dose dependently increased expression of COX-2 production. CONCLUSIONS: UP decreased cell viability, increased the inflammatory response, and increased mucin gene production in HMEECs. These findings indicate that exposure to UP can contribute to the development of otitis media.
OBJECTIVES: The aim of this study was to examine the cytotoxic effect and inflammatory response of human middle ear epithelial cells (HMEECs) induced by urban particles (UP). MATERIALS AND METHODS: Cell viability following UP exposure was assessed in HMEECs using the CCK 8 assay. The expression levels of the inflammation-related genes (COX-2 and MUC5AC) were analyzed using semi-quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and COX-2 production was analyzed using western blotting. RESULTS: Treatment with UP decreased cell viability in HMEECs in a dose- and time-dependent manner. UP exposure induced the significantly increased expression of COX-2 and MUC5AC. Western blotting showed dose dependently increased expression of COX-2 production. CONCLUSIONS: UP decreased cell viability, increased the inflammatory response, and increased mucin gene production in HMEECs. These findings indicate that exposure to UP can contribute to the development of otitis media.
Authors: Jizhen Lin; Lena Hafrén; Joseph Kerschner; Jian-Dong Li; Steve Brown; Qing Y Zheng; Diego Preciado; Yoshihisa Nakamura; Qiuhong Huang; Yan Zhang Journal: Otolaryngol Head Neck Surg Date: 2017-04 Impact factor: 3.497